Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ru-labeled oligonucleotides for photoinduced reactions on targeted DNA guanines
Autore:
Ortmans, I; Content, S; Boutonnet, N; Kirsch-De Mesmaeker, A; Bannwarth, W; Constant, JF; Defrancq, E; Lhomme, J;
Indirizzi:
Free Univ Brussels, B-1050 Brussels, Belgium Free Univ Brussels BrusselsBelgium B-1050 els, B-1050 Brussels, Belgium Hoffmann La Roche Ltd, Pharma Res New Technol, CH-4002 Basel, Switzerland Hoffmann La Roche Ltd Basel Switzerland CH-4002 -4002 Basel, Switzerland Univ Grenoble 1, CNRS, LEDSS, F-38041 Grenoble, France Univ Grenoble 1 Grenoble France F-38041 LEDSS, F-38041 Grenoble, France
Titolo Testata:
CHEMISTRY-A EUROPEAN JOURNAL
fascicolo: 9, volume: 5, anno: 1999,
pagine: 2712 - 2721
SICI:
0947-6539(199909)5:9<2712:ROFPRO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
= PTERIDINE-2,4(1H,3H)-DIONE CHROMOPHORE; SOLID-PHASE SYNTHESIS; DOUBLE-STRANDED DNA; ELECTRON-TRANSFER; BATHOPHENANTHROLINE-RUTHENIUM(II) COMPLEXES; DUPLEX DNA; RUTHENIUM(II) COMPLEXES; METAL-COMPLEXES; ENERGY-TRANSFER; NUCLEIC-ACIDS;
Keywords:
DNA oxidation; electron transfer; oligonucleotides; photooxidations; ruthenium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Kirsch-De Mesmaeker, A Free Univ Brussels, CP 160-08,50 Ave FD Roosevelt, B-1050 Brussels, Belgium Free Univ Brussels CP 160-08,50 Ave FD Roosevelt Brussels Belgium B-1050
Citazione:
I. Ortmans et al., "Ru-labeled oligonucleotides for photoinduced reactions on targeted DNA guanines", CHEM-EUR J, 5(9), 1999, pp. 2712-2721

Abstract

As a strategy to synthesize new sequence-specific DNA photoreagents, oligodeoxyribonucleotides bearing a photoreactive [Ru-II(tap)(2)(dip)](2+) complex (tap = 1,4,5,8-tetraazaphenanthrene: dip = 4,7-diphenylphenanthroline) tethered to a central nucleotide base have been prepared and characterized. The resulting Ru-labeled oligonucleotides exhibit absorption and emission properties of the tethered metal complex and bind to complementary single-stranded DNA sequences. The thermal denaturation curves are not significantlyaffected by the chemical attachment of the complex. Steady-state and time-resolved emission data reveal a significant luminescence quenching upon hybridization of the Ru-labeled oligonucleotide with the complementary target strand, if the strand contains guanines. Based on the behavior of the free complex, the quenching process is attributed to a photoinduced electron transfer from the guanines of the target strand. This primary process is related to the formation of photoproduct(s) on the duplex that generate an irreversible photocrosslinking of the two strands. This work constitutes an initial step in the design of sequence-specific photocrosslinking agents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:17:52