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Titolo:
Mid-trimester fetal livers are a rich source of CD34(+/++) cells for transplantation
Autore:
Golfier, F; Barcena, A; Cruz, J; Harrison, M; Muench, MO;
Indirizzi:
Univ Calif San Francisco, Fetal Treatment Ctr Res Lab, Bone Marrow Transplatat Lab, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Surg, Bone Marrow Transplatat Lab, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Mol Med, Bone Marrow Transplatat Lab, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143ancisco, CA 94143 USA
Titolo Testata:
BONE MARROW TRANSPLANTATION
fascicolo: 5, volume: 24, anno: 1999,
pagine: 451 - 461
SICI:
0268-3369(199909)24:5<451:MFLAAR>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; UMBILICAL-CORD BLOOD; VERSUS-HOST-DISEASE; BONE-MARROW TRANSPLANTATION; IN-UTERO TRANSPLANTATION; HIGH-DOSE CHEMOTHERAPY; EX-VIVO EXPANSION; PROGENITOR CELLS; PERIPHERAL-BLOOD; LONG-TERM;
Keywords:
fetal tissue transplantation; hematopoietic stem cells; liver; CD34(+) cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Muench, MO Univ Calif San Francisco, Fetal Treatment Ctr Res Lab, Bone Marrow Transplatat Lab, 513 Parnassus Ave,Room 1602 HSW, San Francisco, CA 94143 USA Univ Calif San Francisco 513 Parnassus Ave,Room 1602 HSW San Francisco CA USA 94143
Citazione:
F. Golfier et al., "Mid-trimester fetal livers are a rich source of CD34(+/++) cells for transplantation", BONE MAR TR, 24(5), 1999, pp. 451-461

Abstract

Human fetal livers (FL), between 16 and 24 weeks of gestation, were studied for their potential as a source of hematopoietic stem cells for prenatal and postnatal transplantation. In this report we give a quantitative evaluation of human FL as a source of candidate stem cells, and develop a protocol for the isolation of these cells free of microbial contaminants and almost free of mature T cells. Human FLs contained a median 1.9 x 10(9) viable cells and a mean of 1.3 x 10(8) CD34(+/++) cells (range 1.1 x 10(7) to 4.7 x10(8)). Regardless of gestational age, no significant differences mere apparent in the numbers of total progenitors or in the numbers of candidate stem cells (CD34(++) CD38(-) and CD34(++)CD(4+)), suggesting that the expansion in the liver of the early compartments of hematopoietic progenitors reaches a plateau after the sixteenth week of gestation. Colony-forming units culture (CFU-C) were found to range from 4.1 x 10(6) to 2.5 x 10(7) per FL. Positive selection of FL CD34(++) cells was achieved using the Baxter Isolex 50 device. An average purity of 74% and yield of 29% of CD34(+/++) cells was achieved. T cells were depleted by 99.95%, resulting in a mean of 6.5 x10(3) T cells per processed liver. Analysis of candidate stem cell populations and primitive colony-forming cells (CFC) suggested a preferential enrichment of these cells over the total population of CD34(+/++) cells. Processed CD34(+/++) cells were found to be sterile. In conclusion, purification of FL progenitors between 16 and 24 weeks of gestation results in a large number of early progenitors suitable for irt utero and possibly postnatal transplantation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:09:06