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Titolo:
Promoter elements of vav drive transgene expression in vivo throughout thehematopoietic compartment
Autore:
Ogilvy, S; Metcalf, D; Gibson, L; Bath, ML; Harris, AW; Adams, JM;
Indirizzi:
Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Melbourne, Vic 3050, Australia Royal Melbourne Hosp Melbourne Vic Australia 3050 ne, Vic 3050, Australia
Titolo Testata:
BLOOD
fascicolo: 6, volume: 94, anno: 1999,
pagine: 1855 - 1863
SICI:
0006-4971(19990915)94:6<1855:PEOVDT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITION-EFFECT VARIEGATION; GTP EXCHANGE FACTOR; T-CELL; GENE; MICE; PROTOONCOGENE; REGION; TRANSCRIPTION; TRANSDUCTION; REQUIREMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Adams, JM Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Melbourne, Vic 3050, Australia Royal Melbourne Hosp Melbourne Vic Australia 3050 50, Australia
Citazione:
S. Ogilvy et al., "Promoter elements of vav drive transgene expression in vivo throughout thehematopoietic compartment", BLOOD, 94(6), 1999, pp. 1855-1863

Abstract

To develop a method for targeting expression of genes to the full hematopoietic system, we have used transgenic mice to explore the transcriptional regulation of the vav gene, which is expressed throughout this compartment but rarely outside it. Previously, we showed that a cluster of elements surrounding its promoter could drive hematopoietic-specific expression of a bacterial lacZ reporter gene, but the expression was confined to lymphocytes and was sporadically silenced. Those limitations are ascribed here to the prokaryotic reporter gene. With a human CD4 (hCD4) cell surface reporter, thevav promoter elements drove expression efficiently and stably in virtuallyall nucleated cells of adult hematopoietic tissues but not notably in nonhematopoietic cell types. in multiple lines, hCD4 appeared on most, if not all, B and T lymphocytes, granulocytes, monocytes, megakaryocytes, eosinophils, and nucleated erythroid cells. Moreover, high levels appeared on both lineage-committed progenitors and the more primitive preprogenitors. In the fetus, expression was evident in erythroid cells of the definitive but not the primitive type. These results indicate that a prokaryotic sequence can inactivate a transcription unit and that the vav promoter region constitutes a potent transgenic vector for the entire definitive hematopoietic compartment, (C) 1999 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 10:04:29