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Titolo:
A simple method to express soluble, highly active cyclodextrin glycosyltransferase in recombinant E-coli
Autore:
Han, NS; Tao, BY;
Indirizzi:
Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 Biol Engn, W Lafayette, IN 47907 USA
Titolo Testata:
BIOTECHNOLOGY TECHNIQUES
fascicolo: 9, volume: 13, anno: 1999,
pagine: 631 - 635
SICI:
0951-208X(199909)13:9<631:ASMTES>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; PURIFICATION;
Keywords:
cyclodextrin; enzyme over-production; glycosyl transferase; inclusion body; low temperature expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Tao, BY Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 n, W Lafayette, IN 47907 USA
Citazione:
N.S. Han e B.Y. Tao, "A simple method to express soluble, highly active cyclodextrin glycosyltransferase in recombinant E-coli", BIOTECH TEC, 13(9), 1999, pp. 631-635

Abstract

The activity of soluble cyclodextrin glycosyltransferase was increased by 40% without formation of inclusion bodies by induction at low temperature with the addition of 10 mM CaCl2 into the medium, when the corresponding gene was expressed in two recombinant E. coli. Furthermore, the protein expressed at 25 degrees C had approximately 30% higher specific activity than that expressed at 30 degrees C. The two different hosts, promoters, and media showed the same result, indicating that this simple method can be used in expressing other proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 13:55:30