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Titolo:
Cloning and functional characterization of the bovine endothelin-converting enzyme-1a promoter
Autore:
Orzechowski, HD; Richter, CM; Funke-Kaiser, H; Lemmer, J; Theis, S; Paul, M;
Indirizzi:
Free Univ Berlin, Benjamin Franklin Med Ctr, Inst Clin Pharmacol & Toxicol, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 Toxicol, D-12200 Berlin, Germany
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 3, volume: 1446, anno: 1999,
pagine: 352 - 358
SICI:
0167-4781(19990903)1446:3<352:CAFCOT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPRESSION; CELLS; ECE-1; METALLOPROTEASE; ISOFORMS; FAMILY;
Keywords:
endothelin-converting enzyme; promoter; transcription factor; reporter gene assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Paul, M Free Univ Berlin, Benjamin Franklin Med Ctr, Inst Clin Pharmacol &Toxicol, Hindenburgdamm 30, D-12200 Berlin, Germany Free Univ Berlin Hindenburgdamm 30 Berlin Germany D-12200 Germany
Citazione:
H.D. Orzechowski et al., "Cloning and functional characterization of the bovine endothelin-converting enzyme-1a promoter", BBA-GENE ST, 1446(3), 1999, pp. 352-358

Abstract

Endothelin-converting enzyme-1 (ECE-1) mRNA is expressed in three isoforms, termed a, b, and c, originating from alternative promoters. In cultured bovine aortic endothelial cells, we detected mRNA isoform expression of ECE-1a and ECE-1b/c, respectively. Investigating transcriptional mechanisms of bovine endothelial ECE-1a expression in more detail, we identified multipletranscription start sites localized 120-415 nucleotides upstream from the presumptive translation start codon by RNase protection assay and 5' RACE. Using luciferase reporter gene assays we found that 1.4 kb of the 5' untranslated region showed strong promoter activity in endothelial cells. Sequence analysis revealed 71% overall homology of the bovine ECE-1a promoter withits human homologue. The proximal 680 base pair promoter region was shown to contain cis elements that are sufficient for basal and serum-induced transcriptional activation. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 10:13:59