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Titolo:
Phosphatidylinositol 3-kinase is required for growth factor-induced amino acid uptake by vascular smooth muscle cells
Autore:
Higaki, M; Shimokado, K;
Indirizzi:
Natl Cardiovasc Ctr, Inst Res, Suita, Osaka 5658565, Japan Natl CardiovascCtr Suita Osaka Japan 5658565 Suita, Osaka 5658565, Japan
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 9, volume: 19, anno: 1999,
pagine: 2127 - 2132
SICI:
1079-5642(199909)19:9<2127:P3IRFG>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSPORTER GENE-EXPRESSION; ANGIOTENSIN-II; FACTOR RECEPTOR; PROTEIN-SYNTHESIS; SYSTEM-L; INSULIN; INHIBITOR; LY294002; GLUCOSE; METABOLISM;
Keywords:
phosphatidylinositol 3-kinase; amino acid uptake; platelet-derived growth factor; transforming growth factor-beta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Shimokado, K Natl Cardiovasc Ctr, Inst Res, 5-7-1 Fujishirodai, Suita, Osaka 5658565, Japan Natl Cardiovasc Ctr 5-7-1 Fujishirodai Suita Osaka Japan 5658565
Citazione:
M. Higaki e K. Shimokado, "Phosphatidylinositol 3-kinase is required for growth factor-induced amino acid uptake by vascular smooth muscle cells", ART THROM V, 19(9), 1999, pp. 2127-2132

Abstract

Although accumulating evidence suggests that phosphatidylinositol 3-kinase(PI3K) is a common signaling molecule for growth factor-induced amino aciduptake by the cell, the role of PI3K in the uptake of different amino acids was not tested under the same conditions. In this study, we asked whetherPI3K mediates platelet-derived growth factor (PDGF) -stimulated uptake of different amino acids that are taken up through 3 major amino acid transporters expressed in rat vascular smooth muscle cells and other cell types andwhether PI3K mediates amino acid uptake stimulated with different growth factors and vasoactive substances. PDGF increased the uptake of [H-3]leucine, [H-3]proline, and [H-3]arginine in a dose- and time-dependent fashion. Two different PI3K inhibitors, wortmannin (100 nmol/L) and LY294002 (10 mu mol/L), completely inhibited the amino acid uptake stimulated by PDGF. Chinese hamster ovary cells expressing both PDCF receptor-beta and a dominant-negative PI3K did not increase their leucine uptake when stimulated with PDGF,whereas the same cells expressing only PDGF receptor-beta did. Transforming growth factor-beta, as well as insulin-like growth factor-I and angiotensin II, increased leucine uptake by vascular smooth muscle cells. Wortmanninand LY294002 inhibited this increase. We also found that transforming growth factor-beta stimulated PI3K activity and the phosphorylation of Akt, a downstream signaling molecule of PI3K. A similar effect of PI3K inhibitors on amino acid uptake was observed in Swiss 3T3 cells. We conclude that P13K mediates the uptake of different amino acids by vascular smooth muscle cells and other eel types stimulated with a variety of growth factors, including transforming growth factor-beta, Our findings suggest that PI3K may play an important role in vascular pathophysiology by regulating amino acid uptake.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 06:45:16