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Titolo:
SEQUENCING AND IMMUNOCHEMICAL CHARACTERIZATION OF THE AMERICAN COCKROACH PER-A-3 (CR-PI) ISOALLERGENIC VARIANTS
Autore:
WU CH; LEE MF; WANG NM; LUO SF;
Indirizzi:
TAICHUNG VET GEN HOSP,DEPT MED RES,160 CHUNG KANG RD,SECT 3 TAICHUNG TAIWAN CHANG GUNG MEM HOSP,DEPT INTERNAL MED,SECT RHEUMATOL ALLERGY & IMMUNOL TAIPEI 10591 TAIWAN
Titolo Testata:
Molecular immunology
fascicolo: 1, volume: 34, anno: 1997,
pagine: 1 - 8
SICI:
0161-5890(1997)34:1<1:SAICOT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAGWEED POLLEN ALLERGEN; RYE-GRASS POLLEN; AMB-A-II; MAJOR ALLERGENS; MONOCLONAL-ANTIBODIES; CLONING; PROTEIN; RECOMBINANT; ISOFORMS; IGE;
Keywords:
CDNA CLONING; AMERICAN COCKROACH; PER A 3 ALLERGEN; ISOALLERGENIC VARIANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
C.H. Wu et al., "SEQUENCING AND IMMUNOCHEMICAL CHARACTERIZATION OF THE AMERICAN COCKROACH PER-A-3 (CR-PI) ISOALLERGENIC VARIANTS", Molecular immunology, 34(1), 1997, pp. 1-8

Abstract

Two additional members of the American cockroach (Periplaneta americana) Per a 3 (Cr-PI) allergen, C13 and C28, were isolated and sequenced. They encoded proteins of 470 and 393 amino acids with two and no potential N-glycosylation sites, respectively. The molecular weights for C13 and C28 cloned proteins are 56 200 and 46 7000, with PI values of 7.06 and 6.54. C13 and C28 display 95.4% identify with several overlapping predicted central antigenic determinants. Both allergens were also found to have a 95% sequence homology with previously cloned C20 andshare similar antigenic determinants, as defined by the structural prediction and ELISA analysis. However, the recombinant C13 and C28 allergens showed 26.3 and 94.7% skin reactivities on asthmatic patients while C20 elicited 47.4%. While no sequence similarity was found to other known allergens, these two aromatic amino acid-rich allergens were highly related to insect hemolymph proteins (28.7-36.5%), as with C20 cloned protein. Results suggest that these two are isoallergenic variants of C20. Sequence variations among isoforms, resulting a significantdifference in skin reactivities, will be useful in elucidating the allergenic determinants. (C) 1997 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:32:00