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Titolo:
Role of N gamma-acetyldiaminobutyrate as an enzyme stabilizer and an intermediate in the biosynthesis of hydroxyectoine
Autore:
Canovas, D; Borges, N; Vargas, C; Ventosa, A; Nieto, JJ; Santos, H;
Indirizzi:
Univ Sevilla, Fac Farm, Dept Microbiol & Parasitol, E-41012 Seville, SpainUniv Sevilla Seville Spain E-41012 l & Parasitol, E-41012 Seville, Spain Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780 Oeiras, Portugal Univ Nova Lisboa Oeiras Portugal P-2780 & Biol, P-2780 Oeiras, Portugal
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 9, volume: 65, anno: 1999,
pagine: 3774 - 3779
SICI:
0099-2240(199909)65:9<3774:RONGAA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIUM HALOMONAS-ELONGATA; HALOPHILIC EUBACTERIA; COMPATIBLE SOLUTES; STRESS; ECTOINE; MANNOSYLGLYCERATE; ACCUMULATION; TEMPERATURE; BETAINE; CHOLINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Nieto, JJ Univ Sevilla, Fac Farm, Dept Microbiol & Parasitol, E-41012 Seville, Spain Univ Sevilla Seville Spain E-41012 tol, E-41012 Seville, Spain
Citazione:
D. Canovas et al., "Role of N gamma-acetyldiaminobutyrate as an enzyme stabilizer and an intermediate in the biosynthesis of hydroxyectoine", APPL ENVIR, 65(9), 1999, pp. 3774-3779

Abstract

Strain CHR63 is a salt-sensitive mutant of the moderately halophilic wild-type strain Halomonas elongata DSM 3043 that is affected in the ectoine synthase gene (ectC). This strain accumulates large amounts of N gamma-acetyldiaminobutyrate (NADA), the precursor of ectoine (D. Canovas, C. Vargas, F. Iglesias-Guerra, L. N. Csonka, D. Rhodes, A. Ventosa, and J. J. Nieto, J. Biol. Chem. 272:25794-23801, 1997). Hydroxyectoine, ectoine, and glucosylglycerate were also identified by nuclear magnetic resonance (NMR) as cytoplasmic organic solutes in this mutant. Accumulation of NADA, hydroxyectoine, and ectoine was osmoregulated, whereas the levels of glucosylglycerate decreased at higher salinities. The effect of the growth stage on the accumulation of solutes was also investigated. NADA was purified from strain CHR63 and was shown to protect the thermolabile enzyme rabbit muscle lactate dehydrogenase against thermal inactivation. The stabilizing effect of NADA was greater than the stabilizing effect of ectoine or potassium diaminobutyrate. A H-1 NMR analysis of the solutes accumulated by the wild-type strain and mutants CHR62 (ectA::Tn1732) and CHR63 (ectC::Tn1732) indicated that H. elongata can synthesize hydroxyectoine by two different pathways-directly from ectoine or via an alternative pathway that converts NADA into hydroxyectoine without the involvement of ectoine.

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Documento generato il 29/11/20 alle ore 03:23:26