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Titolo:
Development of rabbit embryos during a 96-h period of in vitro culture after superovulatory treatment under conditions of elevated ambient temperature
Autore:
Cheng, H; Dooley, MP; Hopkins, SM; Anderson, LL; Yibchok-anun, S; Hsu, WH;
Indirizzi:
Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA Iowa State Univ Ames IA USA 50011 Univ, Dept Anim Sci, Ames, IA 50011 USA Iowa State Univ, Coll Vet Med, Dept Vet Clin Sci, Ames, IA 50011 USA Iowa State Univ Ames IA USA 50011 , Dept Vet Clin Sci, Ames, IA 50011 USA Iowa State Univ, Coll Vet Med, Dept Biomed Sci, Ames, IA 50011 USA Iowa State Univ Ames IA USA 50011 ed, Dept Biomed Sci, Ames, IA 50011 USA
Titolo Testata:
ANIMAL REPRODUCTION SCIENCE
fascicolo: 3-4, volume: 56, anno: 1999,
pagine: 279 - 290
SICI:
0378-4320(19990816)56:3-4<279:DOREDA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-STRESS; LUTEINIZING-HORMONE; MOUSE OOCYTES; OVARIAN-FUNCTION; BOVINE OOCYTES; MATURATION; INVITRO; SECRETION; RECOVERY; ESTRUS;
Keywords:
gonadotropins; elevated ambient temperature; embryonic development; rabbit;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Anderson, LL Iowa State Univ, Dept Anim Sci, 2356 Kildee Hall, Ames, IA 50011 USA Iowa State Univ 2356 Kildee Hall Ames IA USA 50011 50011 USA
Citazione:
H. Cheng et al., "Development of rabbit embryos during a 96-h period of in vitro culture after superovulatory treatment under conditions of elevated ambient temperature", ANIM REPROD, 56(3-4), 1999, pp. 279-290

Abstract

The effects of elevated ambient temperature on the response to exogenous gonadotropins were evaluated in female New Zealand White rabbits exposed to 33 +/- 1 degrees C (mean +/- SE) and 10-30% relative humidity (8 h/day) during a 5-day period. Does were treated with pFSH (0.3 mg/0.3 mi Standard Armour) twice daily during three consecutive days with a minimum interval of 8h between injections. Six hours after the last FSH injection all does wereremoved from the experimental chamber, given hCG (25 IU/kg) and paired overnight. Nineteen hours after pairing, embryos were flushed from the reproductive tracts, evaluated, and subjected to in vitro culture during a 96-h period. The ovulatory responses to exogenous gonadotropins and fertilization rates did not differ significantly under conditions of elevated ambient temperature, whereas fewer blastocysts and increased number of degenerate embryos were observed after culture. We conclude that although hyperthermia wasinduced during exposure to elevated ambient temperature, it did not alter the ovulatory responses to gonadotropin treatment and plasma concentrationsof FSH and LH compared with does in a thermoneutral environment. Exposure of donor rabbits to elevated ambient temperature before mating, however, increased embryonic degeneration. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 21/09/20 alle ore 01:43:17