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Titolo:
Distribution of AMPA receptor subunits GluR1-4 in the dorsal vagal complexof the rat: A light and electron microscope immunocytochemical study
Autore:
Kessler, JP; Baude, A;
Indirizzi:
CNRS, UPR 9024, Neurobiol Lab, F-13402 Marseille 20, France CNRS Marseille France 20 24, Neurobiol Lab, F-13402 Marseille 20, France
Titolo Testata:
SYNAPSE
fascicolo: 1, volume: 34, anno: 1999,
pagine: 55 - 67
SICI:
0887-4476(199910)34:1<55:DOARSG>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEUS-TRACTUS-SOLITARIUS; NON-NMDA RECEPTORS; SELECTIVE GLUTAMATE RECEPTORS; BRAIN-STEM PROJECTIONS; AMINO-ACID RECEPTORS; AFFERENT SYNAPTIC TRANSMISSION; IN-VIVO MICRODIALYSIS; AREA POSTREMA; MOTOR NUCLEUS; VAGUS NERVE;
Keywords:
glutamate receptors; subcellular distribution; nucleus tractus solitarii; area postrema; autonomic regulations;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
90
Recensione:
Indirizzi per estratti:
Indirizzo: Kessler, JP CNRS, UPR 9024, Neurobiol Lab, 31 Chem Joseph Aiguier, F-13402Marseille 20, France CNRS 31 Chem Joseph Aiguier Marseille France 20 le 20, France
Citazione:
J.P. Kessler e A. Baude, "Distribution of AMPA receptor subunits GluR1-4 in the dorsal vagal complexof the rat: A light and electron microscope immunocytochemical study", SYNAPSE, 34(1), 1999, pp. 55-67

Abstract

The dorsal vagal complex, localized in the dorsomedial medulla, includes the nucleus tractus solitarii (NTS), the dorsal motor nucleus of the vagus nerve (DMN) and the area postrema (AP). The distribution of AMPA-preferring glutamate receptors (AMPA receptors) within this region was investigated using immunohistochemistry and antibodies recognizing either one (GluR1 or GluR4) or two (GluR2 and GluR3) AMPA receptors subunits. The distribution of GluR1 immunoreactivity showed high contrast of staining between strongly and lightly labeled areas. Labeling was intense in the AP and weak in the NTS, except for its medial and dorsalmost parts which exhibited moderate staining. Almost no GluR1 immunoreactivity was found in theDMN. GluR2/3 immunolabeling was present in the entire dorsal vagal complex. This labeling was strong in the AP, the DMN and the medial half of the NTS and moderate in the lateral half of the NTS, except for the interstitial subdivision which exhibited intense staining. Labeling induced by the GluR4antibody was very weak throughout the dorsal vagal complex. Ultrastructural examination showed that GluR1 and GluR2/3 immunoreactivitywas localized in neuronal cell bodies and dendrites. No labeled axon terminal or glial cell body was found. Immunoperoxidase staining in labeled cellbodies and dendrites was associated with intracellular organelles (microtubules, mitochondria, cisternae of the endoplasmic reticulum, ...) and/or parts of the plasma membrane. Plasma membrane labeling was often associated with asymmetrical synaptic differentiations. No labeled symmetrical synapse was found using either GluR1 or GluR2/3 antibody. The present results show that AMPA receptors have a widespread distribution in neuronal perikarya and dendrites of the rat dorsal vagal complex. Theysuggest differences in subunit composition between AMPA receptors localized in the NTS, the DMN and the AP. Ultrastructural data are consistent with the fact that AMPA receptors associated with the plasma membrane are mostlysynaptic receptors. However, they also suggest the existence of a large intracellular pool of receptor subunits in neuronal soma and dendrites. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 14:32:48