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Titolo:
Stage-specific effects of defined mixtures of polychlorinated biphenyls onin vitro development of rabbit preimplantation embryos
Autore:
Kuchenhoff, A; Eckard, R; Buff, K; Fischer, B;
Indirizzi:
Univ Halle Wittenberg, Dept Anat & Cell Biol, Fac Med, D-06097 Halle, Germany Univ Halle Wittenberg Halle Germany D-06097 Med, D-06097 Halle, Germany Univ Munster, Environm Specimen Bank Human Tissue, D-4400 Munster, GermanyUniv Munster Munster Germany D-4400 uman Tissue, D-4400 Munster, Germany GSF, Res Ctr Environm & Hlth, Dept Cell Chem, Oberschleissheim, Germany GSF Oberschleissheim Germany Dept Cell Chem, Oberschleissheim, Germany
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 2, volume: 54, anno: 1999,
pagine: 126 - 134
SICI:
1040-452X(199910)54:2<126:SEODMO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARYL-HYDROCARBON RECEPTOR; REPRODUCTIVE TOXICITY; CHLORINATED HYDROCARBONS; RISK ASSESSMENT; AH RECEPTOR; PCBS; MICE; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN; 2,2',4,4',5,5'-HEXACHLOROBIPHENYL; DIOXINS;
Keywords:
PCB; xenobiotics; blastocysts; embryo toxicity; arylhydrocarbon receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Fischer, B Univ Halle Wittenberg, Dept Anat & Cell Biol, Fac Med, Grosse Steinstr 52,D-06097 Halle, Germany Univ Halle Wittenberg Grosse Steinstr 52 Halle Germany D-06097
Citazione:
A. Kuchenhoff et al., "Stage-specific effects of defined mixtures of polychlorinated biphenyls onin vitro development of rabbit preimplantation embryos", MOL REPROD, 54(2), 1999, pp. 126-134

Abstract

PCBs adversely affect various reproductive functions. Little is known about the embryotoxic effects during the preimplantation period in mammals. In the present study the effects of various mixtures of highly purified PCB-congeners on embryo morphology, blastocyst formation, embryo size and cell proliferation were investigated. For 24 hr, day 3 morulae and day 4 blastocysts were cultured in the presence/absence of coplanar congeners (PCB77, PCB126, and PCB169), noncoplanar congeners (PCB28, PCB52,PCB101,PCB118,PCB138,PCB153,PCB180 or both mixtures in concentrations ranging from 0.3 ng/mL to 60 mu g/mL total PCB. The main effects were (1) degeneration of all embryos at 60 mu g/mL, (2) reduction of cell proliferation in day 4 embryos only bycoplanar PCB; in day 3 embryos, however, by all PCB-mixtures, and (3) reduction of cell proliferation in a non-linear dose response with the strongest impairment caused by the lowest concentration. Cell proliferation was decreased by 0.3 ng/mL coplanar PCB to 50% of the level in control blastocysts. Our results show that purified PCB congeners in the range of 0.3 ng/mL to30 mu g/mL affect the development of preimplantation embryos in a stage-specific and congener-specific manner. This study provides first evidence foran embryotoxic potential of coplanar PCB congeners. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:45:05