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Titolo:
Striatal metabolism of hexanal, a lipid peroxidation product, in the rat
Autore:
Jaar, V; Ste-Marie, L; Montgomery, JA;
Indirizzi:
Univ Montreal, Dept Nutr, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 tr, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
METABOLIC BRAIN DISEASE
fascicolo: 2, volume: 14, anno: 1999,
pagine: 71 - 82
SICI:
0885-7490(199906)14:2<71:SMOHAL>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHROMATOGRAPHY MASS-SPECTROMETRY; HYDROXYL RADICAL GENERATION; PARKINSONS-DISEASE; 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE TOXICITY; 1-METHYL-4-PHENYLPYRIDINIUM ION; OXIDATIVE STRESS; BRAIN INJURY; ALDEHYDES; MPTP; MICRODIALYSIS;
Keywords:
hexanal; acyloin; lipid peroxidation; striatum; pyruvate dehydrogenase; MPP+;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Montgomery, JA CHUM Res Ctr, Neurobiol Lab, Campus Notre Dame,1560 Sherbrooke St E, Montreal, PQ H2L 4M1, Canada CHUM Res Ctr Campus Notre Dame,1560 Sherbrooke St E Montreal PQ Canada H2L 4M1
Citazione:
V. Jaar et al., "Striatal metabolism of hexanal, a lipid peroxidation product, in the rat", METAB BRAIN, 14(2), 1999, pp. 71-82

Abstract

Free radical induced lipid peroxidation may play a role in neurodegeneration and peroxidation leads to the formation of hexanal from omega-6 fatty acids. We have previously demonstrated in vitro that pyruvate dehydrogenase (PDH) catalyzes the condensation of saturated aldehydes with pyruvate to form acyloins. We have further shown in perfused rat heart that hexanal, presumably via PDH, is converted to 3-hydroxyoctan-2-one and that it in turn canbe reduced to 2,3-octanediol. We now extend this work using intra-striatalmicrodialysis to show that this reaction also occurs in rat brain. The reduction of hexanal to hexanol was also evaluated. Microdialysis probes were implanted bilaterally in the striatum and were infused with hexanal with and in the absence of added pyruvate. Analysis of microdialysis samples showed a release of 3-hydroxyoctan-2-one (9.5-10.5 pmol/min), 2,3-octanediol (2.2-2.7 pmol/min) and hexanol (64-74 pmol/min). Pyruvate addition did not increase 3-hydroxyoctan-2-one or 2,3-octanediol production. In a second seriesof experiments where no exogenous hexanal was infused, endogenous production of 3-hydroxyoctan-2-one (1.0-1.3 pmol/min) and 2,3-octanedioi (1.0-1.2 pmol/min) was still observed, although no hexanol was detected. We also investigated the possibility that oxidative stress induced by 1-methyl-4-phenylpyridinium (MPP+) would increase lipid peroxidation resulting in increased production of 3-hydroxyoctan-2-one. Analysis of samples collected followingMPP+ infusion indicated no additional increase suggesting that brief exposure to MPP+ does not increase hexanal formation over baseline levels duringthe experimental period.

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Documento generato il 10/07/20 alle ore 12:49:17