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Titolo:
Susceptibility of bovine naked 2-and 4-cell embryos and hatched blastocysts produced in vitro to infection with noncytopathogenic bovine viral diarrhea virus
Autore:
Tsuboi, T; Imada, T;
Indirizzi:
Natl Inst Anim Hlth, Lab Microbiol Diag, Dept Systemat Diag, Ibaraki, Osaka 3050856, Japan Natl Inst Anim Hlth Ibaraki Osaka Japan 3050856 aki, Osaka 3050856, Japan Natl Inst Anim Hlth, Lab Viral Pathogenesis, Dept Virol, Ibaraki, Osaka 3050856, Japan Natl Inst Anim Hlth Ibaraki Osaka Japan 3050856 aki, Osaka 3050856, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 8, volume: 61, anno: 1999,
pagine: 943 - 945
SICI:
0916-7250(199908)61:8<943:SOBN24>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUCOSAL DISEASE; CATTLE; REPLICATION; CELLS; BORN;
Keywords:
bovine embryo; hatched blastocyst; noncytopathogenic bovine viral diarrhea virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuboi, T Natl Inst Anim Hlth, Lab Microbiol Diag, Dept Systemat Diag, Ibaraki, Osaka 3050856, Japan Natl Inst Anim Hlth Ibaraki Osaka Japan 3050856 3050856, Japan
Citazione:
T. Tsuboi e T. Imada, "Susceptibility of bovine naked 2-and 4-cell embryos and hatched blastocysts produced in vitro to infection with noncytopathogenic bovine viral diarrhea virus", J VET MED S, 61(8), 1999, pp. 943-945

Abstract

To investigate the susceptibility of early bovine embryos to noncytopathogenic bovine viral diarrhea virus (NCP BVDV), 2- and 4-cell embryos producedin vitro from which zona pellucida had been removed by pronase treatment, and hatched blastocysts were exposed to 10(6) TCID50/ml of NCP BVDV No. 12 strain. The virus was detected in all embryo samples immediately prior to cultivation but not in the medium. After 24-hr culture, the virus was isolated from four media and two embryo samples in four experiments in the blastocyst group, and the viral antigen was demonstrated in the cytoplasm of the embryo cells by the immunofluorescent technique. By contrast, no virus was recovered from, or viral antigen detected in samples from the 2- and 4-cellembryo group in any of the experiments, even though they were exposed to the virus after removal of the zona pellucida. These findings suggest that 2- and 4-cell embryos are unlikely to be susceptible to NCP BVDV, but that blastocysts are capable of being infected with the virus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:05:36