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Titolo:
Stimulation of Na+-K+-2Cl(-) cotransport by arsenite in ferret erythrocytes
Autore:
Flatman, PW; Creanor, J;
Indirizzi:
Univ Edinburgh, Sch Med, Dept Biomed Sci Physiol, Membrane Biol Grp, Edinburgh EH8 9AG, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH8 9AG Midlothian, Scotland
Titolo Testata:
JOURNAL OF PHYSIOLOGY-LONDON
fascicolo: 1, volume: 519, anno: 1999,
pagine: 143 - 152
SICI:
0022-3751(19990815)519:1<143:SONCBA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
K-CL COTRANSPORT; INTESTINAL EPITHELIAL-CELLS; PROTEIN-PHOSPHORYLATION; BUMETANIDE BINDING; CALYCULIN-A; RED-CELLS; PHOSPHATASE; TRANSPORT; MAGNESIUM; PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Flatman, PW Univ Edinburgh, Sch Med, Dept Biomed Sci Physiol, Membrane Biol Grp, Teviot Pl, Edinburgh EH8 9AG, Midlothian, Scotland Univ Edinburgh Teviot Pl Edinburgh Midlothian Scotland EH8 9AG
Citazione:
P.W. Flatman e J. Creanor, "Stimulation of Na+-K+-2Cl(-) cotransport by arsenite in ferret erythrocytes", J PHYSL LON, 519(1), 1999, pp. 143-152

Abstract

1. Na+-K+-2Cl(-) cotransport activity was measured in ferret erythrocytes as the bumetanide-sensitive uptake of Rb-86.2. The Na+-K+-2Cl(-) cotransport rate was stimulated by treating erythrocytes with sodium arsenite but not by sodium arsenate (up to 1 mM). Stimulation took an hour to develop fully. Arsenite had no effect on bumetanide-resistant Rb-86 uptake.3. In cells stored for 3 days or less, cotransport stimulation by arsenitecould be described by assuming arsenite either acts at a single site (EC50, 60 +/- 14 mu M, mean + S.E.M., n = 3) or that it acts at both high- (EC50, 35 +/- 9 mu M, mean +/- S.E.M., n = 3) and low- (EC50 > 2 mM) affinity sites.4. Stimulation by 1 mM arsenite tvas greatest on the day of cell collection (rate about 3 times that of the control), even exceeding that produced by20 nM calyculin A, and declined during cell storage, addition of calyculinA to arsenite-stimulated cells resulted in further stimulation of Na+-K+-2Cl(-) cotransport, suggesting that arsenite and calyculin act synergistically. This was most apparent in stored cells.5. Stimulation by 1 mM arsenite was not affected by treating cells with the mitogen-activated protein kinase inhibitors SB203580 (20 mu M) and PD98059 (50 mu M), but was both prevented and reversed by the kinase inhibitors staurosporine (2 mu M) 4-amino-5-(4-methylphenyl)-7-(t-butyl) pyrazolo[3,4-d]pyrimidine (PP1, 50 mu M) and genistein (0.3 mM), and with a combination of 10 mu M A23187 and 2 mM EDTA (to reduce intracellular Mg2+ concentration). Only treatment with EDTA and A23187 prevented stimulation by the combination of 1 mM arsenite and 20 nM calyculin, whereas no treatment was able to fully reverse this stimulation once elicited.6. Our data are consistent with arsenite stimulating (perhaps indirectly) a kinase that phosphorylates and activates the Na+-K+-2Cl(-) cotransporter.

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Documento generato il 02/04/20 alle ore 19:04:51