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Titolo:
Development and analytical characterization of cysteine-grafted polypyrrole films electrosynthesized on Pt- and Ti-substrates as precursors of bioactive interfaces
Autore:
De Giglio, E; Sabbatini, L; Zambonin, PG;
Indirizzi:
Univ Bari, Dipartmento Chim, I-70126 Bari, Italy Univ Bari Bari Italy I-70126 Bari, Dipartmento Chim, I-70126 Bari, Italy
Titolo Testata:
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
fascicolo: 8, volume: 10, anno: 1999,
pagine: 845 - 858
SICI:
0920-5063(1999)10:8<845:DAACOC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLY(LACTIC ACID-CO-LYSINE); CHEMICAL DERIVATIZATION; CELL-ADHESION; PEPTIDE; SURFACE; IMMOBILIZATION; FIBRONECTIN; ATTACHMENT; GROWTH;
Keywords:
polypyrrole; biocompatible polymers; cysteine; surface grafting; X-ray photoelectron spectroscopy; bone implants;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Sabbatini, L Univ Bari, Dipartmento Chim, Via Orabona 4, I-70126 Bari, Italy Univ Bari Via Orabona 4 Bari Italy I-70126 70126 Bari, Italy
Citazione:
E. De Giglio et al., "Development and analytical characterization of cysteine-grafted polypyrrole films electrosynthesized on Pt- and Ti-substrates as precursors of bioactive interfaces", J BIOM SC P, 10(8), 1999, pp. 845-858

Abstract

The grafting of cysteine to polypyrrole(PPY)-coated platinum and titanium substrates has been investigated with the aim of developing innovative bioactive materials of interest for bone implants. Polypyrrole has been chosen as the coating polymer because of its suitability to be electrochemically grown directly onto metallic substrates, of any shape and dimension, leadingto remarkably adherent overlayers. The effectiveness of grafting was monitored by X-ray photoelectron spectroscopy (XPS) which showed the presence ofaminoacid residues onto the polymer surface. Information obtained by an accurate curve fitting of significant regions in the spectra (Cls, N1s, and Ols signals) and by a cross-check of peak area ratios, before and after the grafting process, gave evidence that cysteine forms covalent bonds to pyrrole rings, preferentially in B-positions, via the sulfydryl group, leaving both amino and carboxylic functionalities available for further chemistry. The surface density of cysteine residues was evaluated by microgravimetricmeasurements performed by the electrochemical quartz crystal microbalance and was found suitable for the exploitation of these modified surfaces as bioactive systems. Some preliminary results are reported on the adhesion of neonatal rat calvarial osteoblasts onto titanium substrates after coating by a PPY film modified by a polypeptide having cysteine as a terminal residue and containing the Arg-Gly-Asp aminoacid adhesive sequence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 01:59:12