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Titolo:
Temperature-programmed packed capillary liquid chromatography separation with large volume on-column focusing of retinyl esters
Autore:
Molander, P; Thommesen, SJ; Bruheim, IA; Trones, R; Greibrokk, T; Lundanes, E; Gundersen, TE;
Indirizzi:
Univ Oslo, Dept Chem, N-0315 Oslo, Norway Univ Oslo Oslo Norway N-0315Univ Oslo, Dept Chem, N-0315 Oslo, Norway Univ Oslo, Inst Nutr Res, N-0316 Oslo, Norway Univ Oslo Oslo Norway N-0316 iv Oslo, Inst Nutr Res, N-0316 Oslo, Norway
Titolo Testata:
HRC-JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY
fascicolo: 9, volume: 22, anno: 1999,
pagine: 490 - 494
SICI:
0935-6304(199909)22:9<490:TPCLCS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAT-SOLUBLE VITAMINS; REVERSED-PHASE; ULTRAVIOLET DETECTION; 13-CIS-RETINOIC ACID; DEPENDENCE; RETINOIDS; PLASMA; SPEED;
Keywords:
large volume injection; packed capillary LC; temperature programming; retinyl esters; vitamin A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Molander, P Univ Oslo, Dept Chem, POB 1033, N-0315 Oslo, Norway Univ Oslo POB 1033 Oslo Norway N-0315 33, N-0315 Oslo, Norway
Citazione:
P. Molander et al., "Temperature-programmed packed capillary liquid chromatography separation with large volume on-column focusing of retinyl esters", HRC-J HIGH, 22(9), 1999, pp. 490-494

Abstract

A non-aqueous isocratic reversed-phase packed capillary high performance liquid chromatography method for the determination of retinyl esters, utilizing temperature programming and on-column focusing large volume injection, has been developed. The stationary phase material was C-30, and the mobile phase consisted of acetonitrile-dichloromethane (70:30, v/v). A three-step temperature program, starting at 10 degrees C for 10 min, then 1 degrees/min to 30 degrees C, and finally 2.5/min to 70 degrees C, was found most appropriate. Compared to an isothermal separation at 25 degrees C, this temperature program provided improved peak resolution, enhanced peak shapes of thelast eluting compounds, and a reduction of the overall elution time. A mass limit of detection of 27 pg was found with respect to retinyl palmitate, using UV detection with an "U" shaped flow cell at 327 mm. This correspondsto a concentration limit of detection of 2.7 pg/mu L, when utilizing an injection volume of 10 mu L The concentration of retinyl palmitate in arctic seal If ver samples was estimated to be 62.6 mu g/g liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:05:38