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Titolo:
Different promoter usage and multiple transcription initiation sites of the interleukin-1 receptor-related human ST2 gene in UF7 and TM12 cells
Autore:
Iwahana, H; Yanagisawa, K; Ito-Kosaka, A; Kuroiwa, K; Tago, K; Komatsu, N; Katashima, R; Itakura, M; Tominaga, S;
Indirizzi:
Jichi Med Sch, Dept Biochem, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Med, Div Hematol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Sci Univ Tokyo, Fac Sci & Technol, Dept Appl Biol Sci, Tokyo 162, Japan Sci Univ Tokyo Tokyo Japan 162 nol, Dept Appl Biol Sci, Tokyo 162, Japan Univ Tokushima, Sch Med, Otsuka Dept Clin & Mol Nutr, Tokushima, Japan Univ Tokushima Tokushima Japan a Dept Clin & Mol Nutr, Tokushima, Japan
Titolo Testata:
EUROPEAN JOURNAL OF BIOCHEMISTRY
fascicolo: 2, volume: 264, anno: 1999,
pagine: 397 - 406
SICI:
0014-2956(199909)264:2<397:DPUAMT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HELPER T-CELLS; DNA FRAGMENTS; RESPONSIVE GENE; MAST-CELLS; EXPRESSION; PROTEINS; CLONING; T1/ST2; FIBROBLASTS; INDUCTION;
Keywords:
immunoglobulin superfamily; interleukin-1 receptor-related protein; orphan receptor; promoter usage; ST2 gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Tominaga, S Jichi Med Sch, Dept Biochem, 3311-1 Yakushiji, Minami Kawachi,Tochigi 3290498, Japan Jichi Med Sch 3311-1 Yakushiji Minami Kawachi Tochigi Japan 3290498
Citazione:
H. Iwahana et al., "Different promoter usage and multiple transcription initiation sites of the interleukin-1 receptor-related human ST2 gene in UF7 and TM12 cells", EUR J BIOCH, 264(2), 1999, pp. 397-406

Abstract

The ST2 gene encodes receptor-like molecules that are very similar to the type I interleukin-1 receptor. Two distinct types of the ST2 gene products,ST2 (a soluble secreted form) and ST2L (a transmembrane form) are producedby alternative splicing. Here we demonstrate that the human ST2 gene has two alternative promoters followed by distinct noncoding first exons, which are located more than 8 kb apart and are spliced to the common exon 2 containing the translation initiation site. Within 1001 bp upstream of the transcription initiation site of the cloned distal promoter, there are four GATA-1. The main promoter used for the expression of the ST2 gene in UT-7, a human leukaemic cell line, is distinct from that in TM12, a human fibroblastic cell line. Although UT-7 cells use both distal and proximal promoters, the distal promoter is used dominantly for expression of both ST2 and ST2L mRNA. On the other hand, almost all transcription in TM12 cells starts from the proximal promoter. These results contrast with those of former studies on the rat system, in which ST2 and ST2L mRNA were generated by use of the proximal and distal promoters, respectively. Furthermore, UT-7 cells use multiple transcription initiation sites in both the proximal and distal promoters, whereas the transcription of the ST2 gene in TM12 cells starts at a unique site. Intriguingly, these results suggest that ST2 and ST2L proteins have distinct functions in different cells within different biological systems, such as those of growth control, differentiation and immunological responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 01:14:45