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Titolo:
Studies on fertilization of the teleost. II. Nuclear behavior and changes in histone H1 kinase
Autore:
Iwamatsu, T; Shibata, Y; Yamashita, M;
Indirizzi:
Aichi Univ Educ, Dept Biol, Kariya, Aichi 4488542, Japan Aichi Univ Educ Kariya Aichi Japan 4488542 , Kariya, Aichi 4488542, Japan Hokkaido Univ, Dept Mol & Cellular Interact, Div Biol Sci, Grad Sch Sci, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 ci, Sapporo, Hokkaido 060, Japan
Titolo Testata:
DEVELOPMENT GROWTH & DIFFERENTIATION
fascicolo: 4, volume: 41, anno: 1999,
pagine: 473 - 482
SICI:
0012-1592(199908)41:4<473:SOFOTT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURATION-PROMOTING FACTOR; PROTEIN-SYNTHESIS INHIBITION; SEA-URCHIN EGG; CELL-CYCLE; DNA-SYNTHESIS; MOUSE EGGS; M-PHASE; 6-DIMETHYLAMINOPURINE 6-DMAP; PARTHENOGENETIC ACTIVATION; PRONUCLEAR FORMATION;
Keywords:
fertilization; histone H1 kinase; medaka egg; nuclear behavior; protein synthesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Iwamatsu, T Aichi Univ Educ, Dept Biol, Kariya, Aichi 4488542, Japan AichiUniv Educ Kariya Aichi Japan 4488542 chi 4488542, Japan
Citazione:
T. Iwamatsu et al., "Studies on fertilization of the teleost. II. Nuclear behavior and changes in histone H1 kinase", DEVELOP GR, 41(4), 1999, pp. 473-482

Abstract

In order to understand the dynamic responses of gamete nuclei upon fertilization in the fish, Oryzias latipes, the relationship between changes in the activity of histone H1 kinase and nuclear behavior was examined during fertilization. Kinase activity rapidly decreased concomitant with the initiation of the propagative exocytosis of cortical alveoli following sperm attachment to the egg plasma membrane post-insemination (PI). Activity again increased 30min PI. Similar changes in kinase activity, migration and syngamy of pronuclei, and subsequent cleavage were observed with aphidicolin or actinomycin D treatment, except that formation of abnormal metaphase chromosomes was retarded in aphidicolin-treated zygotes. Pretreatment of unfertilized eggs with cycloheximide or 6-dimethylaminopurine (6-DMAP) caused no nuclear changes. The activity of histone H1 kinase in these eggs rapidly declined following sperm penetration and exocytosis, but did not undergo subsequent increase in the presence of these inhibitors. In these eggs with low histone H1 kinase activity, the fertilization process from sperm penetration tosyngamy occurred normally, but the pronuclear membrane did not break down and the chromosomes did not condense. The present data suggest that in fisheggs, DNA replication as well as the synthesis and phosphorylation of proteins, especially cyclin B, are required for normal formation of metaphase chromosomes at the first cleavage, but not for fertilization events from sperm penetration through to nuclear migration resulting in syngamy.

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Documento generato il 30/11/20 alle ore 17:02:59