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Titolo:
Evaluation of a particle-enhanced turbidimetric immunoassay for the measurement of immunoglobulin E in an ILab 900 analyzer
Autore:
Guaita, S; Simo, JM; Ferre, N; Joven, J; Camps, J;
Indirizzi:
Hosp Univ de Sant Joan, Ctr Recerca Biomed, Reus 43201, Catalunya, Spain Hosp Univ de Sant Joan Reus Catalunya Spain 43201 43201, Catalunya, Spain
Titolo Testata:
CLINICAL CHEMISTRY
fascicolo: 9, volume: 45, anno: 1999,
pagine: 1557 - 1561
SICI:
0009-9147(199909)45:9<1557:EOAPTI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
FERRITIN; SYSTEMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Camps, J Hosp Univ de Sant Joan, Ctr Recerca Biomed, Carrer Sant Joan S-N,Reus 43201, Catalunya, Spain Hosp Univ de Sant Joan Carrer Sant Joan S-N Reus Catalunya Spain 43201
Citazione:
S. Guaita et al., "Evaluation of a particle-enhanced turbidimetric immunoassay for the measurement of immunoglobulin E in an ILab 900 analyzer", CLIN CHEM, 45(9), 1999, pp. 1557-1561

Abstract

Background: The measurement of immunoglobulin E (IgE) in serum is widely used in the diagnosis of allergic reactions and parasitic infections. We describe here a fully automated assay for human IgE suitable for routine application in a general chemistry analyzer. Methods: We used an ILab 900(R) analyzer. This instrument automates a particle-enhanced immunoturbidimetric assay with an analysis time of 9 min. Results: The assay was linear in the range 4-1000 KIU/L (r = 0.9998). The intra- and interassay CVs at 57, 235, and 434 kIU/L were <3.5% and <7.4%, respectively. The detection limit was 4 kIU/L. Hemoglobin (less than or equal to 16 g/L), bilirubin (less than or equal to 250 mu mol/L), and myeloma paraproteins did not interfere with the assay. The assay showed good correlation with a microparticle enzyme immunoassay (r = 0.998) with a mean difference between methods of -6 +/- 26 kIU/L. Conclusion: The new automated serum assay for IgE is an attractive alternative that avoids the need for dedicated instrumentation. (C) 1999 American Association for Clinical Chemistry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 13:20:38