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Titolo:
PROTECTION OF HU-PBL-SCID BEIGE MICE FROM HIV-1 INFECTION BY A 6-MER MODIFIED OLIGONUCLEOTIDE, R-95288/
Autore:
AGATSUMA T; ABE K; FURUKAWA H; KOGA R; KOIZUMI M; HOTODA H; KANEKO M;
Indirizzi:
SANKYO CO LTD,BIOL RES LABS,SHINAGAWA KU,2-58 HIROMACHI 1 CHOME TOKYO140 JAPAN SANKYO CO LTD,ANALYT & METAB RES LABS,SHINAGAWA KU TOKYO 140 JAPAN SANKYO CO LTD,NEW LEAD RES LABS,SHINAGAWA KU TOKYO 140 JAPAN
Titolo Testata:
Antiviral research
fascicolo: 3, volume: 34, anno: 1997,
pagine: 121 - 130
SICI:
0166-3542(1997)34:3<121:POHBMF>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS; SCID MICE; MONOCLONAL-ANTIBODY; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDE; IN-VITRO; GP120; INHIBITION; MECHANISM; AIDS;
Keywords:
OLIGONUCLEOTIDE; HIV-1; IMMUNODEFICIENT MOUSE; SCID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
T. Agatsuma et al., "PROTECTION OF HU-PBL-SCID BEIGE MICE FROM HIV-1 INFECTION BY A 6-MER MODIFIED OLIGONUCLEOTIDE, R-95288/", Antiviral research, 34(3), 1997, pp. 121-130

Abstract

We analyzed the anti-HIV-1 activity of an oligonucleotide derivative,R-95288, in severe combined immunodeficient (SCID/beige) mice transplanted with normal human peripheral blood leukocytes (PBLs), designatedhu-PBL-SCID/beige mice, The human chimeric mice were inoculated with HIV-1(CC1) 3 weeks after the transplantation and sacrificed 2 weeks later. Virus infection was determined by coculture of splenocytes with fresh human PBLs and also by detection of HIV- specific DNA sequences using the polymerase chain reaction. No evidence of infection was observed in mice treated with R-95288 (100 mg/kg/day) using intraperitonealdelivery by osmotic minipumps starting 1 day before virus challenge. In contrast, virus infection was observed in over 80% of the saline-treated control mice. In addition, partial inhibition of HIV-1 infectionwas obtained in mice treated subcutaneously with R-95288 (100 mg/kg/day). Toxicity towards the engrafted human cells was not observed by flow cytometric analysis. Moreover, R-95288 failed to inhibit lymphocyteproliferation (CC50 > 400 mu g/ml), while 90% inhibition of HIV-1 replication was achieved at 3.1 mu g/ml in vitro. These results suggest the ability of R-95288 to protect the human chimeric mice against HIV-1infection. (C) 1997 Elsevier Science B.V.

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Documento generato il 27/09/20 alle ore 06:21:56