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Titolo:
Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: A new biological activity
Autore:
Hirabayashi, K; Yano, J; Inoue, T; Yamaguchi, T; Tanigawara, K; Smyth, GE; Ishiyama, K; Ohgi, T; Kimura, K; Irimura, T;
Indirizzi:
Nippon Shinyaku Co Ltd, Discovery Res Labs, Minami Ku, Kyoto 6018550, Japan Nippon Shinyaku Co Ltd Kyoto Japan 6018550 nami Ku, Kyoto 6018550, Japan Univ Tokyo, Grad Sch Pharmaceut Sci, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Sch Pharmaceut Sci, Tokyo 1130033, Japan
Titolo Testata:
CANCER RESEARCH
fascicolo: 17, volume: 59, anno: 1999,
pagine: 4325 - 4333
SICI:
0008-5472(19990901)59:17<4325:IOCCGB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOPTOTIC RAT THYMOCYTES; CYTO-TOXICITY; GENE-TRANSFER; IDENTIFICATION; ACTIVATION; INTERFERON; ACID; DNA; INDUCTION; NUCLEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Hirabayashi, K Nippon Shinyaku Co Ltd, Discovery Res Labs, Minami Ku, 14 Nishinosho Monguchi Cho, Kyoto 6018550, Japan Nippon Shinyaku Co Ltd 14 Nishinosho Monguchi Cho Kyoto Japan 6018550
Citazione:
K. Hirabayashi et al., "Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: A new biological activity", CANCER RES, 59(17), 1999, pp. 4325-4333

Abstract

A complex of polyinosinic-polycytidylic acid [poly(I). poly(C)] and cationic liposome (LIC) inhibited the growth of many tumor cell lines at low concentration in vitro, but poly(I).poly(C) alone had no such antiproliferativeeffect, The IC50 values of LIC against the tumor cells ranged from 0.1 to 1000 ng/ml, LIC had strong cytotoxic effects on malignant cells of epithelial and fibroblastic origin from various tissues and was also effective against Adriamycin-resistant tumor cells. LIC did not significantly affect the growth of lymphoma cells, leukemia cells, normal diploid fibroblasts, or primacy liver cells at concentrations up to 10 mu g/ml. The mechanism of the antiproliferative effect of LIC against malignant cells was the induction of apoptosis, LIC induced the fragmentation of nuclear DNA and the degradation of rRNA in tumor cells. The DNA fragmentation occurred within 1-5 h after the addition of LIC, and both the fragmentation and the inhibition of cancer-cell growth were suppressed by a nuclease inhibitor, In contrast, caspase inhibitors did not affect the antiproliferative activity of LIC, These results suggest that LIC induced apoptosis in malignant cells through the direct activation of nucleases and not through the activation of caspases. LIC reduced the incidence and the size of metastatic liver-cancer tumors in two different mouse metastatic liver-cancer models using human colon carcinoma cells. Histochemical analysis revealed that the KM12-HX cells in the tumor nodules were undergoing apoptosis; therefore, LIC also induced the apoptosis of tumor cells in vivo. In these animal models, LIC caused no observedchanges in normal hepatocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/06/20 alle ore 14:17:08