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Titolo:
Determining the activation time course of synaptic AMPA receptors from openings of colocalized NMDA receptors
Autore:
Kleppe, IC; Robinson, HPC;
Indirizzi:
Univ Cambridge, Physiol Lab, Cambridge CB2 3EG, England Univ Cambridge Cambridge England CB2 3EG Lab, Cambridge CB2 3EG, England
Titolo Testata:
BIOPHYSICAL JOURNAL
fascicolo: 3, volume: 77, anno: 1999,
pagine: 1418 - 1427
SICI:
0006-3495(199909)77:3<1418:DTATCO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY POSTSYNAPTIC CURRENTS; RAT HIPPOCAMPAL SLICES; MOUSE CENTRAL NEURONS; GLUTAMATE RECEPTORS; CORTICAL-NEURONS; VOLTAGE-CLAMP; CELLS; SYNAPSES; CHANNELS; TRANSMISSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Robinson, HPC Univ Cambridge, Physiol Lab, Downing St, Cambridge CB2 3EG, England Univ Cambridge Downing St Cambridge England CB2 3EG England
Citazione:
I.C. Kleppe e H.P.C. Robinson, "Determining the activation time course of synaptic AMPA receptors from openings of colocalized NMDA receptors", BIOPHYS J, 77(3), 1999, pp. 1418-1427

Abstract

Excitatory postsynaptic currents (EPSCs) in most mammalian central neuronshave a fast alpha-amino-3-hydroxy-5-methyl-4-isoazole-proprionic acid (AMPA) receptor-mediated component, lasting a few milliseconds, and a slow N-methl-(D-aspartic acid (NMDA)-receptor-mediated component, lasting hundreds of milliseconds. The time course of the AMPA phase is crucial in the integrative function of neurons, but measuring it accurately is often confounded by cable filtering between the recording electrode and the synapse. We describe a method for recovering the AMPA phase of individual EPSCs by determining the impulse response of the cable filter from single NMDA channel transitions in the slow tails of the same EPSC, then deconvolving the measured AMPA current. Using simulations, we show that filtering of an AMPA conductance transient in a voltage-clamped dendrite behaves in an almost perfectly linear fashion. Expressions are derived for the time course of single channeltransitions and the AMPA phase filtered through a voltage-clamped cable ora single exponential filter, using a kinetic model for AMPA receptor activation. Fitting these expressions to experimental records directly estimatesthe underlying kinetics of the AMPA phase. Example measurements of spontaneous EPSCs in cultured nonpyramidal rat cortical neurons yielded rising time constants of 0.2-0.8 ms, and decay time constants of 1.3-2 ms at 23-25 degrees C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:47:52