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Titolo:
The extracellular matrix in the mouse brain: Its reactions to endo-alpha-N-acetylgalactosaminidase and certain other enzymes
Autore:
Murakami, T; Ohtsuka, A; Su, WD; Taguchi, T; Oohashi, T; Murakami, T; Abe, K; Ninomiya, Y;
Indirizzi:
Okayama Univ, Sch Med, Dept Anat, Fac Med, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 Anat, Fac Med, Okayama 7008558, Japan Okayama Univ, Sch Med, Dept Biochem & Mol Biol, Fac Med, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 Biol, Fac Med, Okayama 7008558, Japan Okayama Univ, Sch Med, Dept Neurol, Fac Med, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 urol, Fac Med, Okayama 7008558, Japan
Titolo Testata:
ARCHIVES OF HISTOLOGY AND CYTOLOGY
fascicolo: 3, volume: 62, anno: 1999,
pagine: 273 - 281
SICI:
0914-9465(199908)62:3<273:TEMITM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERINEURONAL SULFATED PROTEOGLYCANS; PARVALBUMIN-CONTAINING NEURONS; CENTRAL-NERVOUS-SYSTEM; RAT CEREBRAL-CORTEX; HUMAN VISUAL-CORTEX; HYALURONIC-ACID; COLLOIDAL IRON; SPINAL-CORD; NETS; AGGLUTININ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Murakami, T Okayama Univ, Sch Med, Dept Anat, Fac Med, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 8, Japan
Citazione:
T. Murakami et al., "The extracellular matrix in the mouse brain: Its reactions to endo-alpha-N-acetylgalactosaminidase and certain other enzymes", ARCH HIST C, 62(3), 1999, pp. 273-281

Abstract

As our previous studies have indicated, the cingulate cortex of the adult mouse brain contains many neurons with rich cell surface glycoproteins which are linked by collagenous ligands to perineuronal proteoglycans. The present study demonstrated that exclusive incubation with endo-alpha-N-acetylgalactosaminidase abolished the lectin Vicia villosa or Wisteria floribunda agglutinin (VVA or WFA) labeling of the nerve cell surface glycoproteins, while it neither interfered with the cationic iron colloid or aldehyde fuchsin stainings of the perineuronal proteoglycans nor abolished the Gomori's ammoniacal silver impregnation of the collagenous ligands. Double incubationswith endo-alpha-N-acetylgalactosaminidase and collagenase did not eliminate the lectin VVA or WFA labeling of the nerve cell surface glycoproteins, though they did eliminate the cationic iron colloid and aldehyde fuchsin stainings of the perineuronal proteoglycans as well as the Gomori's ammoniacalsilver impregnation of the collagenous ligands. Triple incubations with endo-alpha-N-acetylgalactosaminidase, collagenase, and endo-alpha-N-acetylgalactosaminidase abolished the lectin VVA or WFA labeling of the nerve cell surface glycoproteins, and also eliminated the cationic iron colloid and aldehyde fuchsin stainings of the perineuronal proteoglycans and the Gomori's ammoniacal silver impregnation of the collagenous ligands. These findings indicate that the nerve cell surface glycoproteins or their terminal N-acetylgalactosamines are digested by endo-alpha-N-acetylgalactosaminidase; thesegalactosamines associated with the collagenous ligands or perineuronal proteoglycans are not digested by endo-alpha-N-acetyl-galactosaminidase; and the terminal N-acetylgalactosamines newly exposed by collagenase incubation are digested by this galactosaminidase. It was further demonstrated that hyaluronidase incubation neither digests the collagenous ligands nor revives the lectin VVA or WFA labeling of the nerve cell surface proteoglycans.

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Documento generato il 03/12/20 alle ore 16:02:28