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Titolo:
ANALYSIS OF THE STABILIZATION OF HEN LYSOZYME BY HELIX MACRODIPOLE AND CHARGED SIDE-CHAIN INTERACTION
Autore:
MOTOSHIMA H; MINE S; MASUMOTO K; ABE Y; IWASHITA H; HASIMOTO Y; CHIJIIWA Y; UEDA T; IMOTO T;
Indirizzi:
KYUSHU UNIV 62,GRAD SCH PHARMACEUT SCI,HIGASHI KU FUKUOKA 81282 JAPAN KYUSHU UNIV 62,GRAD SCH PHARMACEUT SCI,HIGASHI KU FUKUOKA 81282 JAPAN
Titolo Testata:
Journal of Biochemistry
fascicolo: 6, volume: 121, anno: 1997,
pagine: 1076 - 1081
SICI:
0021-924X(1997)121:6<1076:AOTSOH>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-HELICES; SECONDARY STRUCTURE; PROTEIN; DIPOLE; STABILITY; RESIDUES;
Keywords:
HEN LYSOZYME; NMR; SITE-DIRECTED MUTAGENESIS; STABILITY; X-RAY CRYSTALLOGRAPHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
H. Motoshima et al., "ANALYSIS OF THE STABILIZATION OF HEN LYSOZYME BY HELIX MACRODIPOLE AND CHARGED SIDE-CHAIN INTERACTION", Journal of Biochemistry, 121(6), 1997, pp. 1076-1081

Abstract

In the N-terminal region of the alpha-helix of the c-type lysozymes, two Asx residues exist at the 18th, and 27th positions, Hen lysozyme has Asp18/Asn27 (18D/27N), and we prepared three mutant lysozymes, Asn18/Asn27 (18N/27N), Asn18/Asp27 (18N/27D), and Asp18/Asp27 (18D/27D). The stability of the wild-type (18D/27N) lysozyme supported the existence of a hydrogen bond between the side chain of Asp18 and the amide group at the NI position in the ct-helix, while the stability of the 18N/27D lysozyme supported the presence of the capping box between the Ser24 (N-cap) and Asp27 residues, Although electrostatic repulsion was observed between Asp18 and Asp27 residues in 18D/27D lysozyme, the dissociation of each residue contributed to stabilizing the B-helix in 18D/27D lysozyme through hydrogen bonding and charge-helix macrodipole interaction, This is the first evidence that two neighboring negative charges at the N-terminus of the helix both increased the stability of the protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 15:04:39