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Titolo:
Transcription map and expression of bovine herpesvirus-1 glycoprotein D inearly region 4 of bovine adenovirus-3
Autore:
Baxi, MK; Babiuk, LA; Mehtali, M; Tikoo, SK;
Indirizzi:
Univ Saskatchewan, Vet Infect Dis Org, Virol Grp, Saskatoon, SK S7N 5E3, Canada Univ Saskatchewan Saskatoon SK Canada S7N 5E3 skatoon, SK S7N 5E3, Canada Transgene SA, Gene Therapy Dept, F-67000 Strasbourg, France Transgene SA Strasbourg France F-67000 Dept, F-67000 Strasbourg, France
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 261, anno: 1999,
pagine: 143 - 152
SICI:
0042-6822(19990815)261:1<143:TMAEOB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
LATE GENE-EXPRESSION; RECOMBINANT ADENOVIRUS; CELL-LINE; TRANSGENE EXPRESSION; OVINE ADENOVIRUS; DNA-REPLICATION; MESSENGER-RNAS; E4; TYPE-3; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Tikoo, SK Univ Saskatchewan, Vet Infect Dis Org, Virol Grp, Saskatoon, SK S7N 5E3, Canada Univ Saskatchewan Saskatoon SK Canada S7N 5E3 K S7N 5E3, Canada
Citazione:
M.K. Baxi et al., "Transcription map and expression of bovine herpesvirus-1 glycoprotein D inearly region 4 of bovine adenovirus-3", VIROLOGY, 261(1), 1999, pp. 143-152

Abstract

Early region 4 (E4) of bovine adenovirus type 3 (BAV-3) was analyzed by Northern blotting, RT-PCR analysis, cDNA sequencing, and S1 nuclease protection assays. The transcriptional map of the E4 region of BAV-3 has marked dissimilarities from those of mouse adenovirus-1, ovine adenovirus-287, and human adenovirus-2, for which the transcriptional maps have been constructed. The E4 region of BAV-3, located between 98.6 and 89.8 MU transcribes sevendistinct classes of bovine adenovirus type 3 mRNA. The seven mRNA species formed by the removal of one to three introns share both the 3' end and a short 5' leader (25 nucleotides). The E4 mRNAs can encode at least five unique polypeptides, namely, 143R1, 69R, 143R2, 268R, and 219R. Isolation of a replication-competent recombinant "BAV404" containing 1.9-kb insertion [glycoprotein (gD) of bovine herpesvirus 1, under the control of a SV40 early promoter and poly(A)] in the region between E4 and the right ITR suggested that this region is nonessential for BAV-3 replication. Expression of go by BAV404 recombinant virus was confirmed by immunoprecipitation with go-specific monoclonal antibodies. Analysis of the kinetics of protein expression indicated that go is expressed at both early and late limes postinfection, These results suggest that: (a) 54 produces seven 5'-3' coterminal mRNAs and(b) the right terminal region of BAV-3 can be used for the expression of vaccine antigens. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 11:01:44