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Titolo:
Established cell lines and primary cultures in testing medical devices in vitro
Autore:
Cenni, E; Ciapetti, G; Granchi, D; Arciola, CR; Savarino, L; Stea, S; Montanaro, L; Pizzoferrato, A;
Indirizzi:
Ist Ortoped Rizzoli, Lab Biocompatibil Res Implant Mat, Bologna, Italy IstOrtoped Rizzoli Bologna Italy tibil Res Implant Mat, Bologna, Italy
Titolo Testata:
TOXICOLOGY IN VITRO
fascicolo: 4-5, volume: 13, anno: 1999,
pagine: 801 - 810
SICI:
0887-2333(199908/10)13:4-5<801:ECLAPC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ENDOTHELIAL-CELLS; IN-VITRO; PROTEIN EXPRESSION; DACRON; GRAFTS; BONE; BIOCOMPATIBILITY; CYTOTOXICITY; RELEASE; ASSAY;
Keywords:
cell culture; cell function; biomaterial; device;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Cenni, E Ist Ortoped Rizzoli, Lab Biocompatibil Res Implant Mat, Via di Barbiano 1-10, Bologna, Italy Ist Ortoped Rizzoli Via di Barbiano 1-10 Bologna Italy a, Italy
Citazione:
E. Cenni et al., "Established cell lines and primary cultures in testing medical devices in vitro", TOX VITRO, 13(4-5), 1999, pp. 801-810

Abstract

In testing for the cytotoxicity of medical devices, crucial parameters arethe type of cells, the duration of the exposure, the physical form of the device and the method of evaluation. Using cell cultures the changes of cell functions induced by artificial materials may be evaluated. In screening rests the effect of biomaterials on functions common to all cell types is investigated through both continuous lines and primary cultures. In supplementary tests the effect of biomaterials on specific functions is studied, employing cells of the same type of those which will be in contact with the device in vivo. Osteoblasts, either From primary culture or an established line, are used to study the interaction with materials for bone implants. Weevaluated whether dental cements affect the replication cycle of osteoblast-like cells MG63. The S phase was inhibited by the cements with a phenolicgroup, even though to a different extent. Mononuclear cell cultures are used to investigate the immune response, which is important in the host reaction to implant. We investigated whether the chromium ions released in a biological fluid may induce PBMC to produce cytokines involved in bone resorption. Chromium extract did not impair TNF alpha release significantly, but inhibited IL-6 release significantly in stimulated PBMC. Endothelial cell cultures are useful to the evaluation of materials for vascular grafts. We studied the influence of some types of Dacron on endothelial functions. Collagen-coated Dacron decreased significantly the production of 6-keto-prostaglandin F-1 alpha. Knitted Dacron caused a significant increase of tissue factor, a significant reduction in PECAM-1 and a significant increase in ELAM-1. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 04:07:46