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Titolo:
Improved determination of drug metabolism by perifusion of recombinant V79cells carrying human CYP3A4
Autore:
Gebhardt, R; Lippert, C; Schneider, A; Doehmer, J;
Indirizzi:
Univ Tubingen, Inst Physiol Chem, D-72076 Tubingen, Germany Univ TubingenTubingen Germany D-72076 l Chem, D-72076 Tubingen, Germany Univ Klinikum, Inst Biochem, D-04103 Leipzig, Germany Univ Klinikum Leipzig Germany D-04103 Biochem, D-04103 Leipzig, Germany TU Munchen, Inst Toxikol & Umwelthyg, D-80636 Munich, Germany TU Munchen Munich Germany D-80636 l & Umwelthyg, D-80636 Munich, Germany
Titolo Testata:
TOXICOLOGY IN VITRO
fascicolo: 4-5, volume: 13, anno: 1999,
pagine: 639 - 643
SICI:
0887-2333(199908/10)13:4-5<639:IDODMB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHINESE-HAMSTER CELLS; HUMAN CYTOCHROME-P450 3A4; STABLE EXPRESSION; IN-VITRO; HEPATOCYTES; AFLATOXIN-B1; MUTAGENICITY; ACTIVATION; INHIBITORS; ISOFORMS;
Keywords:
cytochrome P450; drug metabolism; perifusion; testosterone; recombinant cell lines; V79 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Gebhardt, R Univ Tubingen, Inst Physiol Chem, D-72076 Tubingen, Germany Univ Tubingen Tubingen Germany D-72076 076 Tubingen, Germany
Citazione:
R. Gebhardt et al., "Improved determination of drug metabolism by perifusion of recombinant V79cells carrying human CYP3A4", TOX VITRO, 13(4-5), 1999, pp. 639-643

Abstract

Recombinant V79Mz cell lines stably co-expressing human CYP3A4 and human NADPH-cytochrome P450 oxidoreductase were used to compare testosterone metabolism under conventional stationary cultivation and under perifusion. In contrast to stationary culture. steady-state conditions for the production of6 beta-hydoxytestosterone were established in the perifusion system even when different concentrations of testosterone were infused sequentially. As a consequence of these favourable conditions, threefold higher maximal metabolic rates could be measured and kinetic constants could be determined more reproducibly than in stationary culture. The results indicate that CYP3A4shows an autocatalytic reaction kinetic probably due to two binding sites Tor testosterone. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:33:11