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Titolo:
Extraction of apoplastic sap from plant roots by centrifugation
Autore:
Yu, Q; Tang, C; Chen, Z; Kuo, J;
Indirizzi:
Univ Western Australia, Ctr Legumes Mediterranean Agr Soil Sci & Plant Nu,Nedlands, WA 6907, Australia Univ Western Australia Nedlands WA Australia 6907 nds, WA 6907, Australia Univ Western Australia, Ctr Microscopy & Microanal, Nedlands, WA 6907, Australia Univ Western Australia Nedlands WA Australia 6907 nds, WA 6907, Australia S China Agr Univ, Coll Biotechnol, Guangzhou 510642, Peoples R China S China Agr Univ Guangzhou Peoples R China 510642 10642, Peoples R China
Titolo Testata:
NEW PHYTOLOGIST
fascicolo: 2, volume: 143, anno: 1999,
pagine: 299 - 304
SICI:
0028-646X(199908)143:2<299:EOASFP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUPINUS-ANGUSTIFOLIUS L; MALATE-DEHYDROGENASE; CAPILLARY ELECTROPHORESIS; SUNFLOWER LEAVES; PH; CELLS; NUTRITION; NITRATE;
Keywords:
apoplastic sap; capillary electrophoresis; centrifugation; malic dehydrogenase; osmolality; lupin (Lupinus angustifolius); pea (Pisum sativum); roots;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Tang, C Univ Western Australia, Ctr Legumes Mediterranean Agr Soil Sci & Plant Nu,Nedlands, WA 6907, Australia Univ Western Australia Nedlands WA Australia 6907 6907, Australia
Citazione:
Q. Yu et al., "Extraction of apoplastic sap from plant roots by centrifugation", NEW PHYTOL, 143(2), 1999, pp. 299-304

Abstract

A centrifugal method for extracting apoplastic sap from roots of lupin (Lupinus angustifolius) and pea (Pisum sativum) plants, and a method to analyse malic dehydrogenase in the sap using capillary electrophoresis, are described. Osmolality of apoplastic sap was relatively constant at relative centrifugal forces (RCFs) between 600 and 3000 g for lupin, and between 600 and4000 g for pea. Electropherograms of a marker enzyme (malic dehydrogenase)and other components in apoplastic and symplastic saps revealed that contamination occurred at 7000 g. It is suggested that apoplastic sap expelled from plant roots at RCF between 600 and 3000 g is free from symplastic contamination, and is regarded as being of apoplastic origin. The proposed method was used to measure apoplastic pH changes in the plant roots in response to external pH, ammonium, nitrate and vanadate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:47:38