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Titolo:
Detection of tyrosinase mRNA by RT-PCR in the peripheral blood of patientswith advanced metastatic melanoma
Autore:
Alao, JP; Mohammed, MQ; Slade, MJ; Retsas, S;
Indirizzi:
Charing Cross Hosp, Melanoma Unit, London W6 8RF, England Charing Cross Hosp London England W6 8RF ma Unit, London W6 8RF, England Charing Cross Hosp, Dept Med Oncol, Breast Grp, London W6 8RF, England Charing Cross Hosp London England W6 8RF ast Grp, London W6 8RF, England
Titolo Testata:
MELANOMA RESEARCH
fascicolo: 4, volume: 9, anno: 1999,
pagine: 395 - 399
SICI:
0960-8931(199908)9:4<395:DOTMBR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; MALIGNANT-MELANOMA; MESSENGER-RNA; REVERSE-TRANSCRIPTASE; CLINICAL STAGE; CELLS; SURVIVAL; MARKER; CANCER;
Keywords:
circulating melanoma cells; disseminated; melanoma; peripheral blood; RT-PCR; tyrosinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Retsas, S Charing Cross Hosp, Melanoma Unit, Fulham Palace Rd, London W6 8RF, England Charing Cross Hosp Fulham Palace Rd London England W6 8RF gland
Citazione:
J.P. Alao et al., "Detection of tyrosinase mRNA by RT-PCR in the peripheral blood of patientswith advanced metastatic melanoma", MELANOMA RE, 9(4), 1999, pp. 395-399

Abstract

Detection of melanoma cells in the peripheral blood has been facilitated by the reverse transcriptase-polymerase chain reaction (RT-PCR), but their presence is of uncertain importance in the evolution of the disease. We studied the detection of melanoma cells using RT-PCR in the peripheral blood of21 patients, four with regional lymph node metastases (American Joint Committee on Cancer [AJCC] stage III) and 17 with disseminated disease (AJCC stage IV), RNA was extracted from 10 ml of heparinized blood following density gradient centrifugation and converted into cDNA for PCR analysis. Assay sensitivity of 10 cells in 10(7) mononuclear cells and granulocytes obtainedfrom 10 ml of peripheral blood was achieved using the G361 and C32 melanoma cell lines. Tyrosinase mRNA was not detected in control samples from healthy volunteers or patients with non-malignant disease. Six patients (one stage III, five stage IV) tested positive for tyrosinase mRNA (28.6%); with one exception, all patients were receiving chemotherapy at the time of sampling. Of the six positive results, three were from patients who initially tested negative but were subsequently positive after a 3-4 week interval. Thelow detection rates of melanoma cells in the peripheral blood of patients with widely disseminated disease is consistent with recent reports and correlates poorly with the clinical stage of melanoma. This may be partly explained by the clinically observed intermittent and random evolution of melanoma metastases. (C) 1999 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 23:02:03