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Titolo:
Integrating the actin and vimentin cytoskeletons: Adhesion-dependent formation of fimbrin-vimentin complexes in macrophages
Autore:
Correia, I; Chu, D; Chou, YH; Goldman, RD; Matsudaira, P;
Indirizzi:
MIT, Whitehead Inst Biomed Res, Cambridge, MA 02142 USA MIT Cambridge MA USA 02142 ehead Inst Biomed Res, Cambridge, MA 02142 USA MIT, Dept Biol, Cambridge, MA 02142 USA MIT Cambridge MA USA 02142MIT, Dept Biol, Cambridge, MA 02142 USA MIT, Div Bioengn & Environm Hlth, Cambridge, MA 02142 USA MIT Cambridge MA USA 02142 oengn & Environm Hlth, Cambridge, MA 02142 USA Northwestern Univ, Sch Med, Dept Cell & Mol Biol, Evanston, IL 60208 USA Northwestern Univ Evanston IL USA 60208 Mol Biol, Evanston, IL 60208 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 4, volume: 146, anno: 1999,
pagine: 831 - 842
SICI:
0021-9525(19990823)146:4<831:ITAAVC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENT NETWORKS; CROSS-LINKING PROTEINS; TAIL DOMAINS; LIVING CELLS; PLECTIN; ORGANIZATION; IDENTIFICATION; ASSOCIATION; FIBROBLASTS; EXPRESSION;
Keywords:
fimbrin; vimentin; cytoskeleton; adhesion; microfilaments;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Correia, I MIT, Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 USA MIT 9 Cambridge Ctr Cambridge MA USA 02142 bridge, MA 02142 USA
Citazione:
I. Correia et al., "Integrating the actin and vimentin cytoskeletons: Adhesion-dependent formation of fimbrin-vimentin complexes in macrophages", J CELL BIOL, 146(4), 1999, pp. 831-842

Abstract

Cells adhere to the substratum through specialized structures that are linked to the actin cytoskeleton. Recent studies report that adhesion also involves the intermediate filament (IF) and microtubule cytoskeletons, although their mechanisms of interaction are unknown. Here we report evidence for a novel adhesion-dependent interaction between components of the actin and IF cytoskeletons. In biochemical fractionation experiments, fimbrin and vimentin coprecipitate from detergent extracts of macrophages using vimentin- or fimbrin-specific antisera. Fluorescence microscopy confirms the biochemical association. Both proteins colocalized to podosomes in the earliest stages of cell adhesion and spreading. The complex is also found in filopodia and retraction fibers. After detergent extraction, fimbrin and vimentin staining of podosomes, filopodia, and retraction fibers are lost, confirming that the complex is localized to these structures. A 1:4 stoichiometry of fimbrin binding to vimentin and a low percentage (1%) of the extracted vimentin suggest that fimbrin interacts with a vimentin subunit. A fimbrin-binding site was identified in the NH2-terminal domain of vimentin and the vimentin binding site at residues 143-188 in the CH1 domain of fimbrin. Based on these observations, we propose that a fimbrin-vimentin complex may be involved in directing the assembly of the vimentin cytoskeleton at cell adhesionsites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 09:27:20