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Titolo:
The carboxyl terminus of the bacteriophage T4 DNA polymerase contacts its sliding clamp at the subunit interface
Autore:
Alley, SC; Jones, AD; Soumillion, P; Benkovic, SJ;
Indirizzi:
Penn State Univ, Dept Chem, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 m, University Pk, PA 16802 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 35, volume: 274, anno: 1999,
pagine: 24485 - 24489
SICI:
0021-9258(19990827)274:35<24485:TCTOTB>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRUCTURAL-ANALYSES; HOLOENZYME COMPLEX; ACCESSORY PROTEINS; REPLICATION; GENE; PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Benkovic, SJ Penn State Univ, Dept Chem, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 Pk, PA 16802 USA
Citazione:
S.C. Alley et al., "The carboxyl terminus of the bacteriophage T4 DNA polymerase contacts its sliding clamp at the subunit interface", J BIOL CHEM, 274(35), 1999, pp. 24485-24489

Abstract

The location of the interaction of the COOH terminus of the bacteriophage T4 DNA polymerase with its trimeric, circular sliding clamp has been established. A peptide corresponding to the COOH terminus of the DNA polymerase was labeled with a fluorophore and fluorescence spectroscopy used to show that it forms a specific complex with the sliding clamp by virtue of its low K-D value (7.1 +/- 1.0 mu M). The same peptide was labeled with a photoaffinity probe and cross-linked to the sliding clamp. Mass spectrometry of tryptic digests determined the sole linkage point to be Ala-159 on the sliding clamp, an amino acid that lies on the subunit interface. These results demonstrate that the COOH terminus of the DNA polymerase is inserted into the subunit interface of its sliding clamp, thereby conferring processivity to the DNA polymerase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 03:30:00