Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Persistent activation of nuclear factor-kappa B in cultured rat hepatic stellate cells involves the induction of potentially novel Rel-like factors and prolonged changes in the expression of I kappa B family proteins
Autore:
Elsharkawy, AM; Wright, MC; Hay, RT; Arthur, MJP; Hughes, T; Bahr, MJ; Degitz, K; Mann, DA;
Indirizzi:
Univ Southampton, Southampton Gen Hosp, Liver Res Grp, Southampton SO16 6YD, Hants, England Univ Southampton Southampton Hants England SO16 6YD 6 6YD, Hants, England Univ St Andrews, Sch Biomed Sci, St Andrews, Fife, Scotland Univ St Andrews St Andrews Fife Scotland Sci, St Andrews, Fife, Scotland Hannover Med Sch, Dept Gastroenterol & Hepatol, D-3000 Hannover, Germany Hannover Med Sch Hannover Germany D-3000 patol, D-3000 Hannover, Germany Univ Munich, Dept Dermatol, D-8000 Munich, Germany Univ Munich Munich Germany D-8000 Dept Dermatol, D-8000 Munich, Germany
Titolo Testata:
HEPATOLOGY
fascicolo: 3, volume: 30, anno: 1999,
pagine: 761 - 769
SICI:
0270-9139(199909)30:3<761:PAONFB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-ADHESION MOLECULE-1; ITO CELLS; TRANSCRIPTIONAL REGULATION; APOPTOSIS; FIBROSIS; ALPHA; LIVER; PROLIFERATION; PROGRESSION; MECHANISMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Mann, DA Univ Southampton, Southampton Gen Hosp, Liver Res Grp, Level D,S Acad Block,Tremona Rd, Southampton SO16 6YD, Hants, England Univ Southampton Level D,S Acad Block,Tremona Rd Southampton Hants England SO16 6YD
Citazione:
A.M. Elsharkawy et al., "Persistent activation of nuclear factor-kappa B in cultured rat hepatic stellate cells involves the induction of potentially novel Rel-like factors and prolonged changes in the expression of I kappa B family proteins", HEPATOLOGY, 30(3), 1999, pp. 761-769

Abstract

Rat hepatic stellate cells (HSC) cultured in serum-containing medium underwent a rapid (3-hour) classical induction of p50:p65 and p65:p65 nuclear factor-kappa B (NF-kappa B) dimers, Subsequent culturing was associated with prolonged expression of active p50:p65 and persistent induction of a high-mobility NF-kappa B DNA binding complex consisting of potentially novel Rel-like protein(s). Formation of the latter complex was competed for by specific double-stranded oligonucleotides, was up-regulated by treatment of HSCs with tumor necrosis factor alpha (TNF-alpha), and was maintained at basal levels of expression by a soluble HSC-derived factor. An NF-kappa B-responsive CAT reporter gene was highly active in early cultured HSCs but was also trans-activated at a lower but significant level in longer-term cultured cells and could be completely suppressed by expression of dominant negative Ikappa B-alpha. Physiological significance of the lower persistent NF-kappaB activities was also demonstrated by the ability of long-term cultured HSCs to support the activity of the NF-kappa B-dependent human intercellular adhesion molecule-1 (ICAM-1) promoter. Freshly isolated HSCs expressed highlevels of I kappa B-alpha and I kappa B-beta. Culture activation was accompanied by a long-term reduction in levels of I kappa B-alpha With no detectable expression in the nuclear fraction of cells, under these conditions p50:p65 was detected in the nucleus. I kappa B-beta expression was transiently reduced and, upon replenishment, was associated with appearance of a lower-mobility I kappa B-beta antibody-reactive species. Bcl3 expression was absent in freshly isolated HSC but was induced during culturing and became a persistent feature of the activated HSC, Inhibition of NF-kappa B DNA binding activity by gliotoxin was associated with increased numbers of apoptoticcells. We suggest that activation of NF-kappa B in cultured HSC is required for expression of specific genes associated with the activated phenotype such as ICAM-1 and may be antiapoptotic for rat HSCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 17:17:58