Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Functional differences between transplantable human hematopoietic stem cells from fetal liver, cord blood, and adult marrow
Autore:
Holyoake, TL; Nicolini, FE; Eaves, CJ;
Indirizzi:
British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada British Columbia Canc Agcy Vancouver BC Canada V5Z 1L3 BC V5Z 1L3, Canada Univ British Columbia, Dept Med Genet, Vancouver, BC, Canada Univ British Columbia Vancouver BC Canada d Genet, Vancouver, BC, Canada
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 9, volume: 27, anno: 1999,
pagine: 1418 - 1427
SICI:
0301-472X(199909)27:9<1418:FDBTHH>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; IMMUNE-DEFICIENT MICE; EXPANSION IN-VITRO; LONG-TERM CULTURES; BONE-MARROW; MULTILINEAGE HEMATOPOIESIS; IMMUNODEFICIENT MICE; QUANTITATIVE ASSAY; PROGENITOR CELLS; SCID/SCID MICE;
Keywords:
ontogeny; human competitive repopulating unit; NOD/SCID mice; LTC-IC; self-renewal;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Eaves, CJ British Columbia Canc Agcy, Terry Fox Lab, 601 W 10th Ave, Vancouver, BC V5Z 1L3, Canada British Columbia Canc Agcy 601 W 10th Ave Vancouver BC Canada V5Z 1L3
Citazione:
T.L. Holyoake et al., "Functional differences between transplantable human hematopoietic stem cells from fetal liver, cord blood, and adult marrow", EXP HEMATOL, 27(9), 1999, pp. 1418-1427

Abstract

The purpose of this study was to develop a simple assay for quantitating transplantable human lymphomyeloid stem cells (competitive repopulating units [CRU]) to enable comparison among the numbers and types of progeny generated in NOD/SCID mice by such cells from different ontologic sources. Sublethally irradiated NOD/SCID mice were transplanted with varying numbers of CD34(+) cell-enriched suspensions of human fetal liver, cord blood, or adult marrow cells. The types and numbers of human cells present in the marrow ofthe mice were measured 6 to 8 weeks later using flow cytometry, in vitro progenitor assays, and secondary transplant endpoints. Frequencies of human CRU obtained by limiting dilution analysis of mice repopulated 6 to 8 weeksposttransplant were the same when the lymphoid and myeloid progeny of CRU were both detected by specific immunophenotypic endpoints as when in vitro myeloid progenitor assays were used to detect CRU myelopoietic activity. The average output per injected CRU of very primitive cells (CD34(+)CD38(-) cells, LTC-IC, and secondary CRU) was found to be highest for fetal liver CRU and progressively decreased (up to >100-fold) for ontologically older CRU. In contrast, the average output of mature cells was highest for cord blood CRU and lowest for fetal liver CRU, despite equivalent production of intermediate progenitors. Differences in the relative numbers of mature lymphoid, myeloid, and erythroid progeny produced by CRU from different ontologic sources were also seen. Finally, evidence of a transplantable human lymphoid-restricted cell present throughout ontogeny was obtained. A simpler and easier assay for enumerating transplantable human stem cells with lymphomyeloid reconstituting activity has been described, and its specificity and sensitivity validated. The use of this assay has revealed ontogeny-associated differences in a variety of functional attributes of human stem cells proliferating and differentiating in an in vivo, but xenogeneic. setting. (C) 1999 International Society for Experimental Hematology. Published by ElsevierScience Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 23:23:32