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Titolo:
Enhancement of G2 checkpoint function by gelsolin transfection in human cancer cells
Autore:
Sakai, N; Ohtsu, M; Fujita, H; Koike, T; Kuzumaki, N;
Indirizzi:
Hokkaido Univ, Sch Med, Div Gene Regulat, Inst Canc,Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan Hokkaido Univ, Sch Med, Dept Internal Med 2, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 251, anno: 1999,
pagine: 224 - 233
SICI:
0014-4827(19990825)251:1<224:EOGCFB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ADP-RIBOSYLATION FACTOR; RAS-TRANSFORMED CELLS; PHOSPHOLIPASE-C; CYCLE CHECKPOINTS; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; BLADDER-CANCER; GENE-PRODUCT; BINDING; ACTIVATION;
Keywords:
gelsolin; ultraviolet C; G2 checkpoint function; diacylglycerol; cyclin B1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Kuzumaki, N Hokkaido Univ, Sch Med, Div Gene Regulat, Inst Canc,Kita Ku, Kita 15,Nishi7, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Kita 15,Nishi7 Sapporo Hokkaido Japan 0608638 n
Citazione:
N. Sakai et al., "Enhancement of G2 checkpoint function by gelsolin transfection in human cancer cells", EXP CELL RE, 251(1), 1999, pp. 224-233

Abstract

We have previously reported that human gastric (TMK1) and urinary bladder (UMUC2) cancer cell lines show markedly reduced expression of an actin-regulatory protein, gelsolin [S. Moriya et al,. (1994), Int. J. Oncol. 5, 1347-1351, M. Tanaka ct al: (1995), Cancer Res. 55, 3228-3232]. When gelsolin expression is restored by transfection, cancer cells lost tumorigenicity in vivo CME. Tanaka et al. (1995), Cancer Res. 55, 3228-3232]. Here, we show that gelsolin-overexpressing TMK1 and UMUC2 cells are more resistant to WC irradiation. Increased resistance is associated with increases in the proportion of cells in the G2: phase of the cell cycle compared to. similarly treated control neotransfectants. After UVC irradiation, synchronized gelsolin-overexpressing UMUC2 cells had a prolonged S phase followed by delayed G2 accumulation compared to neotransfected UMUC2 cells as determined by cell cycle analysis. The levels of cyclin B1 and cdk1 histone Ill kinase activity in gelsolin transfectants remained low during S and early G2 phase and the production of diacylglycerol induced by WC was reduced in gelsolin transfectants compared to neotransfectants. These observations suggest that gelsolin enhances G2 checkpoint function of cells through lipid metabolism, leading to UVC resistance. Considered together with recent evidence that radiation clastogenesis and chemical carcinogenesis are cell-cycle-dependent, down regulation of gelsolin may lead to the malignant transformation of human gastric or urinary bladder cancers by attenuating G2 checkpoint function. (C)1999 Academic Press.

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Documento generato il 21/09/20 alle ore 02:51:21