Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Restriction fragment length polymorphism analysis of PCR-amplified nifH sequences from wetland plant rhizosphere communities
Autore:
Chelius, MK; Lepo, JE;
Indirizzi:
Univ W Florida, Ctr Environm Diagnost & Bioremediat, Pensacola, FL 32514 USA Univ W Florida Pensacola FL USA 32514 ioremediat, Pensacola, FL 32514 USA
Titolo Testata:
ENVIRONMENTAL TECHNOLOGY
fascicolo: 8, volume: 20, anno: 1999,
pagine: 883 - 889
SICI:
0959-3330(199908)20:8<883:RFLPAO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITROGEN-FIXATION; SALT-MARSH; SPARTINA-ALTERNIFLORA; GENE-SEQUENCES; MICROBIAL DNA; SEDIMENTS; AMPLIFICATION; EXTRACTION; SOIL; POLYMERASE;
Keywords:
microbial diversity; nitrogen fixation; RFLP; nitrogen cycle; bioremediation; ecosystem stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Chelius, MK Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA Univ Wisconsin 1575 Linden Dr Madison WI USA 53706 I 53706 USA
Citazione:
M.K. Chelius e J.E. Lepo, "Restriction fragment length polymorphism analysis of PCR-amplified nifH sequences from wetland plant rhizosphere communities", ENV TECHNOL, 20(8), 1999, pp. 883-889

Abstract

We describe a method to assess the community structure of N-2-fixing bacteria in the rhizosphere. Total DNA was extracted from the macrophytic plantsSpartina alterniflora and Sesbania macrocarpa root zones by bead beating and was purified by CsCl-EtBr gradient centrifugation. The average DNA yieldwas 5.5 mu g g' of soil and was of sufficient purity for PCR amplificationof nifH. [alpha-P-32]dCTP was incorporated into the PCR reaction and nifH PCR products were restriction digested. Restriction Fragment Length polymorphism (RFLP) analysis of the amplified sequences revealed differences in the community structure of N-2-fixing rhizobacteria of the field collected salt marsh plant, Spartina alterniflora, and of a laboratory cultured Sesbania macrocarpa. Soil inoculation experiments were used to determine the efficiency of the methods, and amplified nifH DNA could be detected when 10(4) cells each of-Vibrio natriegens and Azotobacter vinelandii were added per gram of soil. Restriction patterns produced by each species were detected at 10(6) cells g(-1) soil. These results indicate that RFLP analysis of amplified nifH sequences from rhizosphere communities may provide information on species composition and reveal shifts in diversify. By examining populationshifts within functional microbial groups, such as the nitrogen-fixing bacteria, the method should have particular utility in assessing stress effects on polluted ecosystems or on those undergoing bioremedial treatments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 04:48:05