Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The rab7 GTPase controls the maturation of Salmonella typhimurium-containing vacuoles in HeLa cells
Autore:
Meresse, S; Steele-Mortimer, O; Finlay, BB; Gorvel, JP;
Indirizzi:
CNRS Marseille Luminy, Ctr Immunol, INSERM, F-13288 Marseille, France CNRSMarseille Luminy Marseille France F-13288 F-13288 Marseille, France Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1W5, Canada Univ British Columbia Vancouver BC Canada V6T 1W5 ver, BC V6T 1W5, Canada Univ British Columbia, Dept Biochem, Vancouver, BC V6T 1W5, Canada Univ British Columbia Vancouver BC Canada V6T 1W5 ver, BC V6T 1W5, Canada Univ British Columbia, Dept Microbiol, Vancouver, BC V6T 1W5, Canada Univ British Columbia Vancouver BC Canada V6T 1W5 ver, BC V6T 1W5, Canada
Titolo Testata:
EMBO JOURNAL
fascicolo: 16, volume: 18, anno: 1999,
pagine: 4394 - 4403
SICI:
0261-4189(19990816)18:16<4394:TRGCTM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYSOSOMAL MEMBRANE-GLYCOPROTEINS; MANNOSE 6-PHOSPHATE RECEPTORS; ENDOPLASMIC-RETICULUM; ENDOCYTIC PATHWAY; COMPARTMENTS; FUSION; TRANSPORT; PROTEINS; LOCALIZATION; BIOGENESIS;
Keywords:
flow cytometry; lysosome; rab7; Salmonella typhimurium; vacuole;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Meresse, S CNRS Marseille Luminy, Ctr Immunol, INSERM, F-13288 Marseille, France CNRS Marseille Luminy Marseille France F-13288 seille, France
Citazione:
S. Meresse et al., "The rab7 GTPase controls the maturation of Salmonella typhimurium-containing vacuoles in HeLa cells", EMBO J, 18(16), 1999, pp. 4394-4403

Abstract

Following entry into non-phagocytic HeLa cells, the facultative pathogen Salmonella typhimurium survives and replicates within a membrane-bound vacuole, Preceding the initiation of intracellular replication there is a lag phase, during which the bacteria modulate their environment, This phase is characterized by the rapid recycling of early endosomal proteins present on the nascent vacuole followed by the acquisition of a subset of lysosomal proteins. To gain a better understanding of the mechanism of intracellular survival, we have followed the biogenesis of the S.typhimurium-containing vacuole (SCV)in HeLa cells expressing different mutant forms of the small GTPase rab7, We demonstrate that the SCV recruits pre-existing lysosomal glycoproteins (Lgps) in a rab7-dependent manner, without directly interacting withlysosomes, We also show the transient accumulation, in the vicinity of theSCV, of novel rab7- and Lgp-containing vesicles containing very low amounts of cathepsin D, The size of these vesicles is dependent on rab7 activity,suggesting a role for rab7 in their homotypic fusion, Taken together, these results indicate that rab7 regulates SCV biogenesis during the phase characterized by the rapid acquisition of lysosomal proteins, We propose that SCV maturation involves its interaction with rab7/Lgp-containing vesicles which are possible intermediate cargo components of the late endocytic pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:19:21