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Titolo:
Rapid detection of mutated E-cadherin in peritoneal lavage specimens from patients with diffuse-type gastric carcinoma
Autore:
Schuhmacher, C; Becker, KF; Reich, U; Schenk, U; Mueller, J; Siewert, JR; Hofler, H;
Indirizzi:
Tech Univ Muenchen, Klinikum Rechts Isar, Dept Surg, D-81675 Munich, Germany Tech Univ Muenchen Munich Germany D-81675 Surg, D-81675 Munich, Germany Tech Univ Munich, Inst Pathol, D-8000 Munich, Germany Tech Univ Munich Munich Germany D-8000 st Pathol, D-8000 Munich, Germany GSF, Natl Res Ctr Hlth & Environm, Inst Pathol, Neuherberg, Germany GSF Neuherberg Germany lth & Environm, Inst Pathol, Neuherberg, Germany
Titolo Testata:
DIAGNOSTIC MOLECULAR PATHOLOGY
fascicolo: 2, volume: 8, anno: 1999,
pagine: 66 - 70
SICI:
1052-9551(199906)8:2<66:RDOMEI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-MUTATIONS; STOMACH; CANCER; ADENOCARCINOMA; PATTERNS; VIMENTIN; PROGRESS; KERATIN;
Keywords:
gastric carcinoma; diffuse-type; E-cadherin; reverse transcription polymerase chain reaction peritoneal lavage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Schuhmacher, C Tech Univ Muenchen, Klinikum Rechts Isar, Dept Surg, Ismaningerstr 22, D-81675 Munich, Germany Tech Univ Muenchen Ismaningerstr 22 Munich Germany D-81675
Citazione:
C. Schuhmacher et al., "Rapid detection of mutated E-cadherin in peritoneal lavage specimens from patients with diffuse-type gastric carcinoma", DIAGN MOL P, 8(2), 1999, pp. 66-70

Abstract

Tumor cells in abdominal lavage specimens from patients with gastric carcinoma strongly predict subsequent peritoneal metastasis and poor prognosis. Reverse transcription (RT)polymerase chain reaction (PCR) detection of wild-type E-cadherin has been claimed to be superior to conventional cytology for the detection of patients who subsequently develop peritoneal metastases. The present study tested this hypothesis and determined whether or not the detection of mutated, tumor-specific E-cadherin messenger RNA in abdominal lavage specimens serve as a useful diagnostic tool. Preoperative lavage specimens from 52 patients with diffuse-type gastric carcinoma and from 5 patients with benign disease were analyzed by conventional cytology and by RT-PCR for amplification of E-cadherin. Tumor cells were detected by cytologyin 8 (15.3%) of the 52 patients with gastric cancer. The E-cadherin was detected in all 57 samples by RT-PCR. Two of these had abnormal E-cadherin amplification products confirmed to be mutations by direct sequencing, which were identical in the primary tumors. These findings suggest that the detection of wild-type E-cadherin is not sufficiently tumor specific. Also, for diffuse gastric carcinomas with confirmed E-cadherin mutations, detection of mutant E-cadherin by RT-PCR is a potentially valuable method for tumor cell detection in lavage specimens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:35:09