Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cadherin-catenin complexes during zebrafish oogenesis: Heterotypic junctions between oocytes and follicle cells
Autore:
Cerda, J; Reidenbach, S; Pratzel, S; Franke, WW;
Indirizzi:
German Canc Res Ctr, Div Cell Biol, D-69120 Heidelberg, Germany German Canc Res Ctr Heidelberg Germany D-69120 69120 Heidelberg, Germany
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 3, volume: 61, anno: 1999,
pagine: 692 - 704
SICI:
0006-3363(199909)61:3<692:CCDZOH>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
EARLY XENOPUS EMBRYOS; INTERMEDIATE FILAMENT ANCHORAGE; PROTEIN ALPHA-CATENIN; BETA-CATENIN; DESMOSOMAL PLAQUE; DIFFERENTIAL EXPRESSION; BRACHYDANIO-RERIO; MOLECULAR-CLONING; EPITHELIAL-CELLS; OVER-EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Cerda, J German Canc Res Ctr, Div Cell Biol, D-69120 Heidelberg, Germany German Canc Res Ctr Heidelberg Germany D-69120 delberg, Germany
Citazione:
J. Cerda et al., "Cadherin-catenin complexes during zebrafish oogenesis: Heterotypic junctions between oocytes and follicle cells", BIOL REPROD, 61(3), 1999, pp. 692-704

Abstract

During vertebrate oogenesis, the germ cells and associated somatic cells remain connected by a variety of adhering junctional complexes. However, themolecular composition of these cellular structures is largely unknown. To identify the proteins forming the heterotypic adherens junctions between oocytes and follicle cells in the zebrafish (Danio rerio), the cDNAs encodingalpha E-catenin and plakoglobin were isolated. Using these cDNAs, in combination with the previously isolated beta-catenin cDNA, and antibodies specific for alpha- and beta-catenin, plakoglobin, and N- and E-cadherin, we found differences in catenin and plakoglobin gene expression during oogenesis. The immunolocalization of these plaque proteins, as well as of cadherins, in the ovarian follicle indicated an enrichment of alpha- and beta-catenin and of E-cadherin-like protein(s) in the oocyte cortex, notably at sites ofoocyte-follicle cell contacts, suggesting the presence of hitherto unknownheterotypic adherens junctions between these cells. By contrast, plakoglobin and N-cadherin localization was restricted to cell-cell contacts in the follicle cell layer. During oocyte maturation, mRNAs for alpha E- and beta-catenin and plakoglobin accumulated, and all three plaque-forming proteins were stored in unfertilized eggs, either in complexed forms with cadherins or as free cytoplasmic pools. These findings suggest possible roles of these junctional proteins during early embryogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 16:04:36