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Titolo:
Molecular cloning and functional characterization of a human homologue of centaurin-alpha
Autore:
Venkateswarlu, K; Cullen, PJ;
Indirizzi:
Univ Bristol, Sch Med Sci, Dept Biochem, Bristol BS8 1TD, Avon, England Univ Bristol Bristol Avon England BS8 1TD Bristol BS8 1TD, Avon, England
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 262, anno: 1999,
pagine: 237 - 244
SICI:
0006-291X(19990819)262:1<237:MCAFCO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADP-RIBOSYLATION-FACTOR; GUANINE-NUCLEOTIDE-EXCHANGE; GTPASE-ACTIVATING PROTEIN; ZINC-FINGER MOTIF; BINDING-PROTEIN; PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE; SACCHAROMYCES-CEREVISIAE; PLASMA-MEMBRANE; PHOSPHOLIPASE-D; CHOLERA-TOXIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Cullen, PJ Univ Bristol, Sch Med Sci, Dept Biochem, Bristol BS8 1TD, Avon,England Univ Bristol Bristol Avon England BS8 1TD 8 1TD, Avon, England
Citazione:
K. Venkateswarlu e P.J. Cullen, "Molecular cloning and functional characterization of a human homologue of centaurin-alpha", BIOC BIOP R, 262(1), 1999, pp. 237-244

Abstract

We report here the molecular cloning, expression, and characterisation of a human homologue of rat centaurin-alpha, which we have termed centaurin-alpha(1). The cDNA contains a single open reading frame, which encodes a 373-amino-acid protein with a calculated molecular weight of 43,429 Daltons. Centaurin-alpha(1) shows high identity at the amino acid level with the othercentaurin-alpha homologues, p42(IP4) and PIP3BP. Northern analysis revealed that centaurin-alpha(1) expresses as a single 2.5-kb transcript, mainly in the brain. Recombinant centaurin-alpha(1) binds the inositol head group of PtdIns(3,4,5)P-3 and Ins(1,3,4,5)P-4, with high affinity (K-d 139.7 +/- 10.5 nM) and inositol phosphate specificity, consistent with it functioning as a putative PtdIns(3,4,5)P-3 receptor. In keeping with this conclusion, we have shown that GFP-tagged centaurin-alpha(1) recruits to the plasma membrane in a PI 3-kinase-dependent manner and the recruitment is inhibited by the PI 3-kinase inhibitor wortmannin, These results suggest that centaurin-alpha(1) can function as an in vivo PtdIns(3, 4, 5)P-3 receptor. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:49:35