Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Analysis of a 762-bp proximal leptin promoter to drive and control regulation of transgene expression of growth hormone receptor in mice
Autore:
Chen, XL; Hartzell, DL; McGraw, RA; Hausman, GJ; Dean, RG; Lee, K; Baile, CA;
Indirizzi:
Univ Georgia, Dept Anim & Dairy Sci, Athens, GA 30602 USA Univ Georgia Athens GA USA 30602 t Anim & Dairy Sci, Athens, GA 30602 USA Univ Georgia, Dept Physiol & Pharmacol, Athens, GA 30602 USA Univ GeorgiaAthens GA USA 30602 hysiol & Pharmacol, Athens, GA 30602 USA ARS, USDA, Athens, GA 30602 USA ARS Athens GA USA 30602ARS, USDA, Athens, GA 30602 USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 262, anno: 1999,
pagine: 187 - 192
SICI:
0006-291X(19990819)262:1<187:AOA7PL>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
OB GENE-EXPRESSION; BINDING PROTEIN-ALPHA; MOUSE OBESE GENE; PRIMARY CULTURES; ADIPOSE-TISSUE; MESSENGER-RNA; INSULIN; DIFFERENTIATION; ADIPOCYTES; ACTIVATION;
Keywords:
transgenic; leptin; promoter; mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, XL Univ Georgia, Dept Anim & Dairy Sci, Athens, GA 30602 USA Univ Georgia Athens GA USA 30602 Dairy Sci, Athens, GA 30602 USA
Citazione:
X.L. Chen et al., "Analysis of a 762-bp proximal leptin promoter to drive and control regulation of transgene expression of growth hormone receptor in mice", BIOC BIOP R, 262(1), 1999, pp. 187-192

Abstract

Transgenic (TG) mice expressing porcine GH receptor (pGHR) directed by a 762-bp proximal leptin promoter were used to analyze the capability of the promoter to drive and regulate pGHR expression in vivo. Transgene expressionoccurred in inguinal, retroper itoneal, and epididymal/parametrial fat depots in both male and female TG mice, but not in wild type (WT) mice. pGHR transgene was also expressed in liver, heart, kidney, muscle, lung, and brain. Levels of pGHR transgene mRNA were higher in tissues other than adipose tissue. Fasting reduced leptin mRNA levels in adipose; however, pGHR transgene expression was not affected in either adipose or muscle. These results suggest (1) the region between +3 and -759 bp of the leptin promoter is able to drive gene expression in vivo, (2) this region may not be responsible for adipose tissue specificity of leptin expression, and (3) this region may not be responsible for negative regulation of leptin gene expression during fasting. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 14:51:57