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Titolo:
The influence of signal noise on the accuracy of kinetic constants measured by surface plasmon resonance experiments
Autore:
Ober, RJ; Ward, ES;
Indirizzi:
Univ Texas, SW Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas, SW Med Ctr, Ctr Canc Immunobiol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Ctr Canc Immunobiol, Dallas, TX 75235 USA Univ Texas, Ctr Engn Math EC35, Richardson, TX 75083 USA Univ Texas Richardson TX USA 75083 gn Math EC35, Richardson, TX 75083 USA
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 1, volume: 273, anno: 1999,
pagine: 49 - 59
SICI:
0003-2697(19990815)273:1<49:TIOSNO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIOSENSOR TECHNOLOGY; BINDING-KINETICS; LIGAND-BINDING; MASS-TRANSPORT; BIACORE;
Keywords:
kinetic constants; surface plasmon resonance; BIAcore; Cramer Rao lower bound; signal averaging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Ober, RJ Univ Texas, SW Med Ctr, Ctr Immunol, 6000 Harry Hines Blvd, Dallas, TX 75235 USA Univ Texas 6000 Harry Hines Blvd Dallas TX USA 75235 TX 75235 USA
Citazione:
R.J. Ober e E.S. Ward, "The influence of signal noise on the accuracy of kinetic constants measured by surface plasmon resonance experiments", ANALYT BIOC, 273(1), 1999, pp. 49-59

Abstract

An analysis is carried out to investigate the accuracy of kinetic parameters obtained using surface plasmon resonance methodology with a BIAcore instrument. The Cramer Rao lower bound for the least possible variance of an estimator of the kinetic parameters is determined. Using simulations it is shown that the standard least-squares estimation technique provides estimatesthat achieve this bound. The theoretical and simulation results are compared with experimental data obtained from an analysis of the interaction of the myc peptide with the anti-myc antibody, 9E10. This investigation indicates that the accuracy of the results depends on the signal level which has particular relevance to the design of experiments with low signal levels. Itis shown how the accuracy of the estimates of the kinetic constants depends on the kinetic constants themselves and how the accuracy of the association constants depends on the concentration of the analyte that is used in the experiment. In addition, the effects of increasing the number of data points in the analysis of dissociation data on the accuracy of the estimates are quantitated. It is also demonstrated that signal averaging of data derived from repeat sensorgrams can result in a significant decrease in the standard deviation of the estimates. (C) 1999 Academic Press.

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Documento generato il 27/11/20 alle ore 20:57:07