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Titolo:
ECL cell morphology
Autore:
Chen, DA; Zhao, CM; Andersson, K; Meister, B; Panula, P; Hakanson, R;
Indirizzi:
Univ Lund, Dept Pharmacol, S-22362 Lund, Sweden Univ Lund Lund Sweden S-22362 Lund, Dept Pharmacol, S-22362 Lund, Sweden Karolinska Inst, Dept Neurosci, Stockholm, Sweden Karolinska Inst Stockholm Sweden Inst, Dept Neurosci, Stockholm, Sweden Turku Acad Univ, Dept Biol, Turku, Finland Turku Acad Univ Turku Finland urku Acad Univ, Dept Biol, Turku, Finland
Titolo Testata:
YALE JOURNAL OF BIOLOGY AND MEDICINE
fascicolo: 3-4, volume: 71, anno: 1998,
pagine: 217 - 231
SICI:
0044-0086(199805/08)71:3-4<217:ECM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROCHROMAFFIN-LIKE CELLS; ELECTRON-MICROSCOPIC IDENTIFICATION; EVOKED HISTAMINE DEPLETION; GASTRIC-ACID SECRETION; PERFUSED RAT STOMACH; ENDOCRINE-CELLS; ALPHA-FLUOROMETHYLHISTIDINE; CIRCULATING PANCREASTATIN; CHROMOGRANIN-A; ULTRASTRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Hakanson, R Univ Lund, Dept Pharmacol, Solvegatan 10, S-22362 Lund, SwedenUniv Lund Solvegatan 10 Lund Sweden S-22362 2362 Lund, Sweden
Citazione:
D.A. Chen et al., "ECL cell morphology", YALE J BIOL, 71(3-4), 1998, pp. 217-231

Abstract

Using immunohistochemistry at the conventional light, confocal and electron microscopic levels, we have demonstrated that rat stomach ECL cells storehistamine and pancreastatin in granules and secretory vesicles, while histidine decarboxylase occurs in the cytosol. Furthermore the ECL cells display immunoreactivity for vesicular monoamine transporter type 2 (VMAT-2), synaptophysin, synaptotagmin III, vesicle-associated membrane protein-2, cysteine string protein, synaptosomal-associated protein of 25 kDa, syntaxin andMunc-18. Using electron microscopy in combination with stereological methods, we have evidence to suggest the existence of both an exocytotic and a crinophagic pathway in the ECL cells. The process of exocytosis in the ECL cells seems to involve a class of proteins that promote or participate in the fusion between the granule/vesicle membrane and the plasma membrane. The granules take up histamine by VMAT-2 from the cytosol during transport fromthe Golgi zone to the more peripheral parts of the cells. As a result, they turn into secretory vesicles. As a consequence of stimulation (e.g., by gastrin), the secretory vesicles fuse with the cell membrane to release their contents by exocytosis. The crinophagic pathway was studied in hypergastrinemic rats. In the ECL cells of such animals, the secretory vesicles were found to fuse not only with the cell membrane but also with each other to form vacuoles. Subsequent lysosomal degradation of the vacuoles and their contents resulted in the development of lipofuscin bodies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:48:53