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Titolo:
Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system
Autore:
Moon, YH; Kang, HG; Jung, JY; Jeon, JS; Sung, SK; An, G;
Indirizzi:
Pohang Univ Sci & Technol, Dept Life Sci, Pohang 790784, South Korea Pohang Univ Sci & Technol Pohang South Korea 790784 790784, South Korea
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 4, volume: 120, anno: 1999,
pagine: 1193 - 1203
SICI:
0032-0889(199908)120:4<1193:DOTMRF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MADS-BOX GENE; HOMEOTIC GENE; MOLECULAR CHARACTERIZATION; TRANSCRIPTION FACTORS; ECTOPIC EXPRESSION; ANTIRRHINUM-MAJUS; OVULE DEVELOPMENT; ARABIDOPSIS; APETALA3; DOMAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: An, G Pohang Univ Sci & Technol, Dept Life Sci, Pohang 790784, South KoreaPohang Univ Sci & Technol Pohang South Korea 790784 4, South Korea
Citazione:
Y.H. Moon et al., "Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system", PLANT PHYSL, 120(4), 1999, pp. 1193-1203

Abstract

A MADS family gene, OsMADS6, was isolated from a rice (Oryza sativa L.) young flower cDNA library using OsAMDS1 as a probe. With this clone, various MADS box genes that encode far protein-to-protein interaction partners of the OsMADS6 protein were isolated by the yeast two-hybrid screening method. On the basis of sequence homology, OsMADS6 and the selected partners can beclassified in the APETALA1/AGAMOUS-LIKE9 (AP1/AGL9) family. One of the interaction partners, OsMADS14, was selected for further study. Both genes began expression at early stages of flower development, and their expression was extended into the later stages. In mature flowers the OsMADS6 transcriptwas detectable in lodicules and also weakly in sterile lemmas and carpels,whereas the OsMADS14 transcript was detectable in sterile lemmas, paleas/lemmas, stamens, and carpels. Using the yeast two-hybrid system, we demonstrated that the region containing of the 109th to 137th amino acid residues of OsMADS6 is indispensable in the interaction with OsMADS14. Site-directed mutation analysis revealed that the four periodical leucine residues withinthe region are essential for this interaction. Furthermore, it was shown that the 14 amino acid residues located immediately downstream of the K domain enhance the interaction, and that the two leucine residues within this region play an important role in that enhancement.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 04:39:47