Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Embryo cloning by nuclear transfer: experiences in sheep
Autore:
Loi, P; Boyazoglu, S; Fulka, J; Naitana, S; Cappai, P;
Indirizzi:
Ist Zootecn & Caseario Sardegna, I-07040 Olmedo, SS, Italy Ist Zootecn & Caseario Sardegna Olmedo SS Italy I-07040 Olmedo, SS, Italy Univ Perugia, Fac Med Vet, Ist Prod Anim, I-06100 Perugia, Italy Univ Perugia Perugia Italy I-06100 Ist Prod Anim, I-06100 Perugia, Italy Res Inst Anim Prod, Prague, Czech Republic Res Inst Anim Prod Prague Czech Republic m Prod, Prague, Czech Republic Univ Sassari, Fac Med Vet, Ist Biol Anim, I-07100 Sassari, Italy Univ Sassari Sassari Italy I-07100 Ist Biol Anim, I-07100 Sassari, Italy
Titolo Testata:
LIVESTOCK PRODUCTION SCIENCE
fascicolo: 2-3, volume: 60, anno: 1999,
pagine: 281 - 294
SICI:
0301-6226(19990715)60:2-3<281:ECBNTE>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSPLANT RABBIT EMBRYOS; CELL-CYCLE; 6-DIMETHYLAMINOPURINE 6-DMAP; BOVINE EMBRYOS; IN-VITRO; OOCYTES; REPLICATION; CALCIUM; STAGE; EGG;
Keywords:
sheep; embryos; nuclear transfer; cloning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Loi, P Ist Zootecn & Caseario Sardegna, I-07040 Olmedo, SS, Italy Ist Zootecn & Caseario Sardegna Olmedo SS Italy I-07040 SS, Italy
Citazione:
P. Loi et al., "Embryo cloning by nuclear transfer: experiences in sheep", LIVEST PROD, 60(2-3), 1999, pp. 281-294

Abstract

Although technically established in mammalian species more than 15 year ago, the efficiency of embryo cloning by nuclear transfer in terms of offspring production has been invariably low. This article reviews a series of interrelated experiments carried out from 1992 to 1997 regarding the co-ordination of nuclear and cytoplasmic events after embryo reconstruction. The experiments were undertaken at the Istituto Zootecnico e Caseario per la Sardegna (IZCS) and the sheep used belonged to the Sardinian dairy breed. The use of enucleated, pre-activated recipient cytoplasts resulted in an increased frequency of development to blastocyst of embryos reconstructed with unsynchronized blastomere nuclei. This increase in development was due to the absence of chromatin damage and unbalanced ploidy in nuclei transferred after the decay of MPF (Maturation Promoting Factor), The combination of synchronous S-phase cytoplast-karyoplast nuclear transfer together with the reduction of embryo losses from the oviduct of temporary recipients allowed for the first time the production of large numbers of clones of genetically identical lambs, thus bringing nuclear transfer closer to practical application. Finally, the last series of experiments described deals with the establishment of an alternative protocol for embryonic nuclear transfer where the combined use of a chemical activating agent, ionomycin, with a protein kinase inhibitor, 6-Dimethylaminopurine, resulted in the highest development rates to blastocyst stage of metaphase II enucleated oocytes reconstructed with embryonic nuclei described so far.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:37:53