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Titolo:
Constitutive protein expression of monocyte chemotactic protein-1 (MCP-1) by myelomonocytic cell lines and regulation of the secretion by anti- and proinflammatory stimuli
Autore:
Steube, KG; Meyer, C; Drexler, HG;
Indirizzi:
Deutsch Sammlung Mikroorganismen & Zellkulturen, Dept Human & Anim Cell Cultures, D-38124 Braunschweig, Germany Deutsch Sammlung Mikroorganismen & Zellkulturen Braunschweig Germany D-38124
Titolo Testata:
LEUKEMIA RESEARCH
fascicolo: 9, volume: 23, anno: 1999,
pagine: 843 - 849
SICI:
0145-2126(199909)23:9<843:CPEOMC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ENDOTHELIAL-CELLS; BLOOD MONONUCLEAR LEUKOCYTES; TUMOR-NECROSIS-FACTOR; AMINO-ACID ANALYSIS; NF-KAPPA-B; CHEMOATTRACTANT PROTEIN-1; ACTIVATING-FACTOR; GENE-EXPRESSION; JE GENE; MALIGNANT-CELLS;
Keywords:
chemokine; MCP-1; monocytic leukemia; cell lines; MONO-MAC-6;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Steube, KG Deutsch Sammlung Mikroorganismen & Zellkulturen, Dept Human & Anim Cell Cultures, Mascheroder Weg 1B, D-38124 Braunschweig, Germany Deutsch Sammlung Mikroorganismen & Zellkulturen Mascheroder Weg 1B Braunschweig Germany D-38124
Citazione:
K.G. Steube et al., "Constitutive protein expression of monocyte chemotactic protein-1 (MCP-1) by myelomonocytic cell lines and regulation of the secretion by anti- and proinflammatory stimuli", LEUK RES, 23(9), 1999, pp. 843-849

Abstract

We have investigated the protein expression of the chemokine monocyte chemotactic/chemoattractant protein-1 (MCP-1) in various human myelomonocytic leukemia cell lines. Applying specific ELISA, we demonstrated that this chemokine is produced constitutively by the cell lines HL-60, ML-2, MONO-MAC-B and MUTZ-3 ranging between 440 and 1400 pg/ml MCP-I per million cells. In the culture medium of two other unstimulated cell lines, MONO-MAC-1 and THP-1, almost no MCP-1 was detected. Stimulation of HL-60 and MONO-MAC-6 with lipopolysaccharide (LPS), and stimulation of ML-2 and MUTZ-3 with 12-tetradecanoyl phorbol 13-acetate (TPA) dramatically increased the MCP-1 level in the culture medium. The highest amount of MCP-1 (> 80 ng/ml within 24 h) wasachieved by TPA stimulation of MUTZ-3 cells. Out of 15 cytokines tested for induction or enhancement of MCP-1 secretion, interleukin-3 (IL-3), IL-6, interferon-gamma (IFN-gamma), granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor (hd-CSF) and tumor necrosis factor (TNF alpha) were able to augment (twofold to 12-fold) the MCP-1 level in the culture medium of MONO-MAC-6 cells. While the antinflammatory cytokines IL-4, IL-10 and IL-13 failed to suppress MCP-1 secretion, the glucocorticoid dexamethasone strongly inhibited the MCP-1 production of unstimulated and stimulated MONO-MAC-6 cells. Thus, several regulatory elements are involved in MCP-1 secretion. Despite the quantitative differences of MCP-1 production among the cell lines analyzed, our results demonstrated a constitutive secretion in differentiation-arrested myelomonocytic leukemia cell lines and emphasize the usefulness of these malignant cell lines as models to study MCP-I secretion and regulation. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 18:45:23