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Titolo:
Phenotypic diversity of enterotoxigenic Escherichia coli strains from a community-based study of pediatric diarrhea in Periurban Egypt
Autore:
Peruski, LF; Kay, BA; Abu El-Yazeed, R; El-Etr, SH; Cravioto, A; Wierzba, TF; Rao, M; El-Ghorab, N; Shaheen, H; Khalil, SB; Kamal, K; Wasfy, MO; Svennerholm, AM; Clemens, JD; Savarino, SJ;
Indirizzi:
USN, Med Res Unit 3, Cairo, Egypt USN Cairo EgyptUSN, Med Res Unit 3, Cairo, Egypt Univ Nacl Autonoma Mexico, Fac Med, Mexico City 04510, DF, Mexico Univ Nacl Autonoma Mexico Mexico City DF Mexico 04510 y 04510, DF, Mexico NICHHD, Bethesda, MD 20892 USA NICHHD Bethesda MD USA 20892NICHHD, Bethesda, MD 20892 USA Univ Gothenburg, Dept Med Microbiol & Immunol, Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden crobiol & Immunol, Gothenburg, Sweden
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 9, volume: 37, anno: 1999,
pagine: 2974 - 2978
SICI:
0095-1137(199909)37:9<2974:PDOEEC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONIZATION FACTOR ANTIGENS; MONOCLONAL-ANTIBODIES; B-SUBUNIT; CHILDREN; VACCINE; DISEASE; SEROTYPES; COUNTRIES; COHORT; HUMANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Peruski, LF USN, Med Res Unit 3, PSC 452,Box 5000, FPO, AE 09835 USA USN PSC 452,Box 5000 FPO AE USA 09835 5000, FPO, AE 09835 USA
Citazione:
L.F. Peruski et al., "Phenotypic diversity of enterotoxigenic Escherichia coli strains from a community-based study of pediatric diarrhea in Periurban Egypt", J CLIN MICR, 37(9), 1999, pp. 2974-2978

Abstract

No past studies of diarrhea in children of the Middle East have examined in detail the phenotypes of enterotoxigenic Escherichia coli (ETEC) strains,which are important pathogens in this setting During a prospective study conducted from November 1993 to September 1995 with 242 children under 3 years of age with diarrhea living near Alexandria, Egypt, 125 episodes of diarrhea were positive for ETEC;ETEC strains were available for 98 of these episodes, from which 100 ETEC strains were selected and characterized on the basis of enterotoxins, colonization factors (CFs), and O:H serotypes. Of these representative isolates, 57 produced heat-stable toxin (ST) only, 34 produced heat-labile toxin (LT) only, and 9 produced both LT and ST. Twenty-three ETEC strains expressed a CF, with the specific factors being CF antigenTV (CFA/IV; 10 of 23; 43%), CFA/II (5 of 23; 22%), CFA/I (3 of 23; 13%), PCFO166 (3 of 23; 13%), and CS7(2 of 23; 9%). No ETEC strains appeared to express CFA/III, CS17, or PCFO159. Among the 100 ETEC strains, 47 O groups and 20 H groups were represented, with 59 O:H serotypes. The most common O serogroups were O159 (13 strains) and O43 (10 strains). O148 and O21 were each detected in five individual strains, O7 and O56 were each detected in four individual strains, O73, O20, O86, and O114 were each detected in three individual strains, and O23, O78, O91, O103, O128, and O132 were each detected in two individual strains. The most common H serogroups were H4 (16 strains), 12 of which were of serogroup O159; H2 (9 strains), all of which wereO43; H18 (6 strains); H30 (6 strains); and H28 (5 strains); strains of thelast three H serogroups were all O148. Cumulatively, our results suggest ahigh degree of clonal diversity of disease-associated ETEC strains in thisregion. As a low percentage of these strains expressed a CF,it-remains possible that other adhesins for which we either did not assay or that are as yet undiscovered are prevalent in this region. Our findings point out some potential barriers to effective immunization against ETEC diarrhea in this population and emphasize the need to identify additional protective antigens commonly expressed by ETEC for inclusion in future vaccine candidates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 18:30:24