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Titolo:
International multicenter evaluation of the clinical utility of a dipstickassay for detection of Leptospira-specific immunoglobulin M antibodies in human serum specimens
Autore:
Smits, HL; Ananyina, YV; Chereshsky, A; Dancel, L; Lai-A-Fat, RFM; Chee, HD; Levett, PN; Masuzawa, T; Yanagihara, Y; Muthusethupathi, MA; Sanders, EJ; Sasaki, DM; Domen, H; Yersin, C; Aye, T; Bragg, SL; Gussenhoven, GC; Goris, MGA; Terpstra, WJ; Hartskeerl, RA;
Indirizzi:
Royal Trop Inst, Dept Biomed Res, NL-1105 AZ Amsterdam, Netherlands Royal Trop Inst Amsterdam Netherlands NL-1105 AZ Amsterdam, Netherlands Russian Acad Med Sci, NF Gamaleya Epidemiol & Microbiol Res Inst, Moscow, Russia Russian Acad Med Sci Moscow Russia & Microbiol Res Inst, Moscow, Russia ESR Hlth Communicable Dis Ctr, Wellington, New Zealand ESR Hlth Communicable Dis Ctr Wellington New Zealand ngton, New Zealand Univ Philippines, Coll Publ Hlth, Dept Med Microbiol, Manila, Philippines Univ Philippines Manila Philippines Med Microbiol, Manila, Philippines Acad Hosp, Dept Dermatol, Paramaribo, Surinam Acad Hosp Paramaribo Surinam d Hosp, Dept Dermatol, Paramaribo, Surinam Acad Hosp, Dept Internal Med, Paramaribo, Surinam Acad Hosp Paramaribo Surinam sp, Dept Internal Med, Paramaribo, Surinam Minist Hlth & Environm, Leptospira Lab, St Michael, Barbados Minist Hlth &Environm St Michael Barbados ra Lab, St Michael, Barbados Univ Shizuoka, Sch Pharmaceut Sci, Dept Microbiol, Shizuoka 422, Japan Univ Shizuoka Shizuoka Japan 422 ci, Dept Microbiol, Shizuoka 422, Japan Chennai Med Coll Tamil Nadu, Dept Nephrol, Chennai, India Chennai Med CollTamil Nadu Chennai India Dept Nephrol, Chennai, India Ctr Dis Control & Prevent, Dengue Branch, Div Vector Borne Infect Dis, SanJuan, PR USA Ctr Dis Control & Prevent San Juan PR USA ne Infect Dis, SanJuan, PR USA Ctr Dis Control & Prevent, Epidemiol Program Off, Atlanta, GA USA Ctr Dis Control & Prevent Atlanta GA USA ol Program Off, Atlanta, GA USA Ctr Dis Control & Prevent, Meningitis & Special Pathogens Branch, Div Bacterial & Mycot Dis, Atlanta, GA USA Ctr Dis Control & Prevent Atlanta GA USA al & Mycot Dis, Atlanta, GA USA Dept Hlth, Epidemiol Branch, Honolulu, HI USA Dept Hlth Honolulu HI USADept Hlth, Epidemiol Branch, Honolulu, HI USA Dept Hlth, Med Microbiol Branch, Pearl City, HI USA Dept Hlth Pearl City HI USA th, Med Microbiol Branch, Pearl City, HI USA Victoria Hosp, Dept Med, Victoria, Seychelles Victoria Hosp Victoria Seychelles Hosp, Dept Med, Victoria, Seychelles
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 9, volume: 37, anno: 1999,
pagine: 2904 - 2909
SICI:
0095-1137(199909)37:9<2904:IMEOTC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; DIAGNOSIS; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Smits, HL Royal Trop Inst, Dept Biomed Res, Meibergdreef 39, NL-1105 AZ Amsterdam, Netherlands Royal Trop Inst Meibergdreef 39 Amsterdam NetherlandsNL-1105 AZ
Citazione:
H.L. Smits et al., "International multicenter evaluation of the clinical utility of a dipstickassay for detection of Leptospira-specific immunoglobulin M antibodies in human serum specimens", J CLIN MICR, 37(9), 1999, pp. 2904-2909

Abstract

We performed a multicenter evaluation of a robust and easily performed dipstick assay for the serodiagnosis of human leptospirosis. The assay is aimed at the detection of Leptospira-specific immunoglobulin M (IgM) antibodies. The study involved 2,665 serum samples collected from 2,057 patients withsuspected leptospirosis in 12 countries on five continents with different levels of endemicity and different surveillance systems. The patients were grouped as laboratory-confirmed leptospirosis case patients and noncase patients based on the results of culturing and the microscopic agglutination test. Paired samples from 27.7% of the subjects were tested. Of the 485 casepatients, 87.4% had a positive dipstick result for one or more samples. Ofthe 1,513 noncase patients, only 7.2% had a positive result. Whereas most (88,4%) of the positive samples from the ease patients showed moderate to strong (2+ to 4+) staining in the dipstick assay, most (68.1%) of the positive samples from the noncase patients showed weak (1+) staining. The sensitivity of the dipstick assay increased from 60.1% far acute-phase serum samples to 87.4% for convalescent-phase samples. The specificities for these twogroups of samples were 94.1 and 92.7%, respectively, The dipstick assay detected a broad variety of serogroups. The results of the dipstick assay were concordant (observed agreement, 93.2%; kappa value, 0.76) with the results of an enzyme-linked immunosorbent assay for the detection of specific IgMantibodies, a test which is often used in the laboratory diagnosis of current or recent leptospirosis. This study demonstrated that this easily performed dipstick assay is a valuable and useful test for the quick screening for leptospirosis; has a wide applicability in different countries with different degrees of endemicity; can be used at all levels of the health care system, including the field; and mill be useful for detecting and monitoringoutbreaks of leptospirosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 17:54:18