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Titolo:
The major olive pollen allergen (Ole e I) shows both gametophytic and sporophytic expression during anther development, and its synthesis and storagetakes place in the RER
Autore:
Alche, JDD; Castro, AJ; Olmedilla, A; Fernandez, MDC; Rodriguez, R; Villalba, M; Rodriguez-Garcia, MI;
Indirizzi:
CSIC, Estac Expt Zaidin, E-18008 Granada, Spain CSIC Granada Spain E-18008 IC, Estac Expt Zaidin, E-18008 Granada, Spain Univ Complutense, Fac Quim, Dept Bioquim & Biol Mol, E-28040 Madrid, SpainUniv Complutense Madrid Spain E-28040 & Biol Mol, E-28040 Madrid, Spain
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 15, volume: 112, anno: 1999,
pagine: 2501 - 2509
SICI:
0021-9533(199908)112:15<2501:TMOPA(>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-MICROSCOPIC LOCALIZATION; PROTEIN-BODY FORMATION; AMINO-ACID-SEQUENCE; AMB-A-I; ENDOPLASMIC-RETICULUM; OLEACEAE POLLENS; BIRCH POLLEN; CYTOPLASMIC NUCLEOLOIDS; SITU HYBRIDIZATION; ANHYDROUS FIXATION;
Keywords:
Ole e I; allergen; pollen; tapetum; expression; Olea europaea L.;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Rodriguez-Garcia, MI CSIC, Estac Expt Zaidin, Profesor Albareda 1, E-18008Granada, Spain CSIC Profesor Albareda 1 Granada Spain E-18008 Spain
Citazione:
J.D.D. Alche et al., "The major olive pollen allergen (Ole e I) shows both gametophytic and sporophytic expression during anther development, and its synthesis and storagetakes place in the RER", J CELL SCI, 112(15), 1999, pp. 2501-2509

Abstract

The distribution of Ole e I (the major olive pollen allergen) and its transcripts was investigated in the anther from premeiotic stages until the dehiscent pollen stage. Crude protein extracts were analyzed by immunoblottingand probed with a monoclonal antibody to Ole e I. The protein, with three variants, was found to accumulate from the early microspore stage onwards. In addition to the previously reported localization of the protein, Ole e Ihas been immunolocalized for the first time within the pollen wall and in the tapetum, Reverse transcription-polymerase chain reaction analysis usingspecific oligonucleotides and RNA extracted from whole anthers revealed that the Ole e I gene is expressed from the late tetrad stage onwards. No expression was found in control tissues such as petals, roots or leaves. Lightmicroscopy in situ hybridization on developing flower buds and dehiscent pollen confirmed the transcripts to be present in both the microspores and the sporophytic tissue (tapetum). Labeling was found primarily in the tapetum, reaching the highest concentration in the cytoplasm of the developing and mature pollen, once tapetum started to degenerate. In situ hybridization at the transmission electron microscope level showed the transcripts to accumulate on ribosomes of the rough endoplasmic reticulum, These studies, together with others carried out previously by us, indicated that both synthesis and storage of Ole e I take place in the endoplasmic reticulum, coincidentally with the conspicuous changes suffered by this membrane system duringpollen development. This process is most likely controlled at the transcriptional level, The localization of the protein in the pollen ectexine bringnew insights into the function of the allergen, which are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:14:05