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Titolo:
Guanidine hydrochloride-induced denaturation of Pseudomonas cepacia lipase
Autore:
Tanaka, A; Okuda, K; Senoo, K; Obata, H; Inouye, K;
Indirizzi:
Mie Univ, Fac Bioresources, Tsu, Mie 5148507, Japan Mie Univ Tsu Mie Japan 5148507 Fac Bioresources, Tsu, Mie 5148507, Japan Kyoto Univ, Grad Sch Agr, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Univ, Grad Sch Agr, Kyoto 6068502, Japan
Titolo Testata:
JOURNAL OF BIOCHEMISTRY
fascicolo: 2, volume: 126, anno: 1999,
pagine: 382 - 386
SICI:
0021-924X(199908)126:2<382:GHDOPC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
OPEN CONFORMATION; ACTIVATION; CHAPERONE; CLONING; ABSENCE;
Keywords:
denaturation; guanidine hydrochloride; lipase from Pseudomonas cepacia; unfolding;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, A Mie Univ, Fac Bioresources, Tsu, Mie 5148507, Japan Mie Univ Tsu Mie Japan 5148507 sources, Tsu, Mie 5148507, Japan
Citazione:
A. Tanaka et al., "Guanidine hydrochloride-induced denaturation of Pseudomonas cepacia lipase", J BIOCHEM, 126(2), 1999, pp. 382-386

Abstract

The guanidine hydrochloride-induced denaturation of Pseudomonas cepacia lipase (PCL) was studied at pH 7 by monitoring the changes in the fluorescence and circular dichroism of the enzyme. The denaturation was irreversible as a whole, and the addition of Ca2+ ions decreased the velocity of the denaturation, The denaturation process was well explained consistently by a two-step mechanism, as follows:[GRAPHICS]where N is the native state of PCL, D-1 an intermediate denatured-state which can be refolded into the native state, and D-F the final denatured-state that can not be renatured. Ethanol (10%) increased the denaturation velocity by decreasing the refolding step, D-1+Ca2+-->N Ca2+, which would be caused by the stabilization of D-1 by ethanol.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:57:46