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Titolo:
Metabolic suppression of platelet-type 12-lipoxygenase in human uterine cervix with invasive carcinoma
Autore:
Nigami, S; Kumar, GS; Sutherland, M; Schewe, T; Ikawa, H; Yamasaki, Y; Ueda, N; Yamamoto, S;
Indirizzi:
Free Univ Berlin, Benjamin Franklin Med Ctr, Eicosanoid Res Div, Gynaecol Dept, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 ol Dept, D-12200 Berlin, Germany Kochi Red Cross Hosp, Kochi, Japan Kochi Red Cross Hosp Kochi JapanKochi Red Cross Hosp, Kochi, Japan Univ Tokushima, Sch Med, Dept Biochem, Tokushima 770, Japan Univ Tokushima Tokushima Japan 770 d, Dept Biochem, Tokushima 770, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 6, volume: 82, anno: 1999,
pagine: 827 - 831
SICI:
0020-7136(19990909)82:6<827:MSOP1I>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROPEROXIDE GLUTATHIONE-PEROXIDASE; MOLECULAR-CLONING; A431 CELLS; EXPRESSION; LIPOXYGENASE; CANCER; 5-LIPOXYGENASE; LOCALIZATION; PROTEIN; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Nigami, S Free Univ Berlin, Benjamin Franklin Med Ctr, Eicosanoid Res Div,Gynaecol Dept, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 -12200 Berlin, Germany
Citazione:
S. Nigami et al., "Metabolic suppression of platelet-type 12-lipoxygenase in human uterine cervix with invasive carcinoma", INT J CANC, 82(6), 1999, pp. 827-831

Abstract

Several types of lipoxygenases with various functions occur in mammalian cells. Although the presence of 12-lipoxygenase activity has been reported in uterine tissues, neither its type nor its biological functions have yet been established. Moreover, the putative role of uterine 12-lipoxygenase in cervical cancer has not been addressed before. Homogenates of uterine tissues from women without cancer and from patients with invasive cervical carcinoma were incubated with (I-C-14)-arachidonic acid under various conditionsand the labelled reaction products were analyzed both by thin-layer chromatography and by high-pressure liquid chromatography. 12-Lipoxygenase protein was estimated by Western blot using anti-serum against recombinant human platelet-type 12-lipoxygenase. Highest concentrations and activities of 12-lipoxygenase were found in the exocervix, The formation of 12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (12-HETE) was stimulated by micromolar concentrations of 13S-hydroperoxy-9Z,11E-octadecadienoic acid, suggesting metabolic control of the 11-lipoxygenase activity via the hydroperoxide tone. Immunohistochemical investigation revealed that the enzyme is mainly located in the squamous epithelium, and is of platelet-type. Significantly lower values for the 12-HETE formation were found in samples from patients with invasive cervical carcinoma, whereas the amount of immunochemically detectable12-lipoxygenase protein was unaltered. At the same time the expression levels of the bcl-2 gene were enhanced. Thus, it is concluded that during carcinogenesis the hydroperoxide-reducing capacity of the uterine cervix tissueis enhanced, possibly mediated by bcl-2 protein, and in turn metabolicallysuppresses the 12-lipoxygenase activity. Furthermore, the data suggest an anti carcinogenic action of 12-lipoxygenase in human cervix, in contrast toits reported pro-carcinogenic action in breast cancer. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 14:29:48